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AB125006

重组Anti-LMAN1抗体[EPR6979]

Anti-LMAN1 antibody [EPR6979]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • 了解详情

5

(4 Reviews)

|

(16 Publications)

Rabbit Recombinant Monoclonal LMAN1 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

查看别名

ERGIC53, F5F8D, LMAN1, Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6979] (AB125006)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling LMAN1 with Purified ab125006 at 1/500 dilution (0.21 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • WB

Lab

Western blot - Anti-LMAN1 antibody [EPR6979] (AB125006)

Lanes 1-4 : Merged signal (red and green). Green - ab125006 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab125006 Anti-LMAN1 antibody [EPR6979] was shown to specifically react with Protein ERGIC-53 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266248 (knockout cell lysate ab257505) was used. Wild-type and Protein ERGIC-53 knockout samples were subjected to SDS-PAGE. ab125006 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°CC at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LMAN1 antibody [EPR6979] (ab125006) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

LMAN1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human LMAN1 knockout HEK-293T cell line (<a href='/products/cell-lines/human-lman1-knockout-hek-293t-cell-line-ab266248'>ab266248</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 58 kDa

Observed band size: 55 kDa

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Western blot - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • WB

Unknown

Western blot - Anti-LMAN1 antibody [EPR6979] (AB125006)

All lanes:

Western blot - Anti-LMAN1 antibody [EPR6979] (ab125006) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse spleen lysate at 20 µg

Lane 4:

Mouse heart lysate at 20 µg

Lane 5:

Rat spleen lysate at 20 µg

Lane 6:

Rat heart lysate at 20 µg

Lane 7:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 58 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6979] (AB125006)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling LMAN1 with Purified ab125006 at 1/500 dilution (0.21 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-LMAN1 antibody [EPR6979] (AB125006)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling LMAN1 with Purified ab125006 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6979] (AB125006)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling LMAN1 with Purified ab125006 at 1/500 dilution (0.21 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody [EPR6979] (AB125006)

Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling LMAN1 with Purified ab125006 at 1/50 dilution (2 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-LMAN1 antibody [EPR6979] (AB125006)
  • WB

CiteAb

Western blot - Anti-LMAN1 antibody [EPR6979] (AB125006)

LMAN1 western blot using anti-LMAN1 antibody [EPR6979] ab125006. Publication image and figure legend from Emmer, B., Hesketh, G. G., et al., 2018, Elife, PubMed 30251625.

ab125006 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab125006 please see the product overview.

SURF4 localizes to the early secretory pathway where it physically interacts with PCSK9.(A) Immunofluorescence of FLAG-SURF4 together with markers of the ER (calnexin), ERGIC (LMAN1), and Golgi (GM130). Scale bar = 10 µm. (B) Quantification of colocalization (n = 8 cells analyzed for each combination of antibody staining). (C) Spectral counts for SURF4 in streptavidin-purified eluates from cells expressing various BirA* fusion proteins. (D) Immunoprecipitations were performed using antibodies directed against FLAG or GFP from lysates of cells expressing FLAG-SURF4, PCSK9-eGFP, both, or neither. Error bars represent standard deviations.

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不同偶联物与剂型 (10)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6979

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>Heat up to 98°C, below boiling, and then let cool for 10-20 min.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LMAN1 also known as ERGIC-53 or lectin mannose-binding 1 is a protein involved in the transport and quality control of glycoproteins. It has a mass of approximately 53 kDa. LMAN1 primarily expresses in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is important for efficient cargo sorting and proper folding. It functions by binding to specific carbohydrate moieties on glycoproteins aiding their transport between the ER and Golgi apparatus.
Biological function summary

LMAN1 functions significantly within a protein complex known as the LMAN1-MCFD2 complex. It serves an essential role as a cargo receptor for glycoproteins particularly those with particular mannose-rich domains influencing their secretion and stability. This protein complex is integral in maintaining protein homeostasis and cellular physiology by ensuring the correct sorting and transport of glycoproteins from the ER to the Golgi.

Pathways

LMAN1 significantly participates in the secretory pathway and quality control mechanisms in the cell. It interacts with proteins such as MCFD2 to mediate the transport of specific glycoproteins like α1-antitrypsin through the secretory pathway. This pathway is important for the secretion of a variety of proteins and disruptions can lead to disorders associated with improper protein folding and trafficking.

Defects in LMAN1 can lead to bleeding disorders such as combined coagulation factor V and VIII deficiency. The protein's dysfunction impairs the transport of coagulation factors disrupting their secretion. Additionally proteins like MCFD2 work closely with LMAN1 in this context as mutations in either protein can cause a similar deficiency highlighting the significant role of LMAN1 in maintaining normal hemostatic processes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
See full target information LMAN1
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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Diabetologia 66:2042-2061 PubMed37537395

2023

SERCA2 regulates proinsulin processing and processing enzyme maturation in pancreatic beta cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hitoshi Iida,Tatsuyoshi Kono,Chih-Chun Lee,Preethi Krishnan,Matthew C Arvin,Staci A Weaver,Timothy S Jarvela,Renato C S Branco,Madeline R McLaughlin,Robert N Bone,Xin Tong,Peter Arvan,Iris Lindberg,Carmella Evans-Molina

Cell & bioscience 12:48 PubMed35477478

2022

Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Edmund Cotter,Ya-Juan Wang,Xu Fu,Angela L Whittsette,Joseph W Lynch,R Luke Wiseman,Jeffery W Kelly,Angelo Keramidas,Ting-Wei Mu

JCI insight 7: PubMed35015735

2022

Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Di Chen,Elizabeth D Hughes,Thomas L Saunders,Jiangping Wu,Magda N Hernandez Vasquez,Taija Makinen,Philip D King

Cellular and molecular gastroenterology and hepatology 13:879-899 PubMed34923175

2021

Hepatic TM6SF2 Is Required for Lipidation of VLDL in a Pre-Golgi Compartment in Mice and Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Luo,Eriks Smagris,Sarah A Martin,Goncalo Vale,Jeffrey G McDonald,Justin A Fletcher,Shawn C Burgess,Helen H Hobbs,Jonathan C Cohen

The Journal of cell biology 221: PubMed34919127

2021

Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargo.

Applications

Unspecified application

Species

Unspecified reactive species

Swathi Devireddy,Shawn M Ferguson

Cellular and molecular gastroenterology and hepatology 12:633-651 PubMed33848642

2021

Mettl14-Mediated mA Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyue Cao,Yuke Shu,Yuwei Chen,Qing Xu,Gang Guo,Zhenru Wu,Mingyang Shao,Yongjie Zhou,Menglin Chen,Yuping Gong,Chuan Li,Yujun Shi,Hong Bu

Scientific reports 11:915 PubMed33441653

2021

Quantitative STAU2 measurement in lymphocytes for breast cancer risk assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Charoenchai Puttipanyalears,Sikrit Denariyakoon,Phonthep Angsuwatcharakon,Vitavat Aksornkitti,Mawin Vongsaisuwan,Sutasinee Asayut,Somchai Thanasitthichai,Narisorn Kongruttanachok,Chatchawit Aporntewan,Apiwat Mutirangura

Cell death and differentiation 28:1733-1752 PubMed33335289

2020

Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Cerrato,Marion Leduc,Kevin Müller,Peng Liu,Liwei Zhao,Juliette Humeau,Wei Xie,Shuai Zhang,Oliver Kepp,Allan Sauvat,Guido Kroemer

Neural regeneration research 16:561-566 PubMed32985488

2020

Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-You Fan,Yi-Lin Pang,Wen-Xiang Li,Chen-Xi Zhao,Yan Zhang,Xu Wang,Guang-Zhi Ning,Xiao-Hong Kong,Chang Liu,Xue Yao,Shi-Qing Feng

The Journal of cell biology 219: PubMed31863584

2019

CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane M Camus,Marine D Camus,Carmen Figueras-Novoa,Gaelle Boncompain,L Amanda Sadacca,Christopher Esk,Anne Bigot,Gwyn W Gould,Dimitrios Kioumourtzoglou,Franck Perez,Nia J Bryant,Shaeri Mukherjee,Frances M Brodsky
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。
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