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AB32583

Anti-LMAN1抗体

Anti-LMAN1 antibody

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Rabbit Polyclonal LMAN1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Rat Lman1 aa 150-200.

查看别名

ERGIC53, F5F8D, LMAN1, Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1

5 Images
Western blot - Anti-LMAN1 antibody (AB32583)
  • WB

Unknown

Western blot - Anti-LMAN1 antibody (AB32583)

All lanes:

Western blot - Anti-LMAN1 antibody (ab32583) at 1/1000 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat brain lysate with Immunising peptide

Predicted band size: 58 kDa

Observed band size: 57.5 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in p19 cells by Immunocytochemistry/Immunofluorescence.

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in HMVEC cells by Immunocytochemistry/Immunofluorescence.

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in A549 cells by Immunocytochemistry/Immunofluorescence.

Western blot - Anti-LMAN1 antibody (AB32583)
  • WB

Supplier Data

Western blot - Anti-LMAN1 antibody (AB32583)

All lanes:

Western blot - Anti-LMAN1 antibody (ab32583) at 1/2000 dilution

Lane 1:

HepG2 lysate at 30 µg

Lane 2:

HeLa lysate at 30 µg

Lane 3:

PANC-1 lysate at 30 µg

Lane 4:

MCF7 lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 58 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Rat, Human, Mouse

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Rat Lman1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q62902

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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您是否考虑以下替代产品？

AB125006

Anti-LMAN1 antibody [EPR6979]

5
4 Reviews
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为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LMAN1 also known as ERGIC-53 or lectin mannose-binding 1 is a protein involved in the transport and quality control of glycoproteins. It has a mass of approximately 53 kDa. LMAN1 primarily expresses in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is important for efficient cargo sorting and proper folding. It functions by binding to specific carbohydrate moieties on glycoproteins aiding their transport between the ER and Golgi apparatus.
Biological function summary

LMAN1 functions significantly within a protein complex known as the LMAN1-MCFD2 complex. It serves an essential role as a cargo receptor for glycoproteins particularly those with particular mannose-rich domains influencing their secretion and stability. This protein complex is integral in maintaining protein homeostasis and cellular physiology by ensuring the correct sorting and transport of glycoproteins from the ER to the Golgi.

Pathways

LMAN1 significantly participates in the secretory pathway and quality control mechanisms in the cell. It interacts with proteins such as MCFD2 to mediate the transport of specific glycoproteins like α1-antitrypsin through the secretory pathway. This pathway is important for the secretion of a variety of proteins and disruptions can lead to disorders associated with improper protein folding and trafficking.

Defects in LMAN1 can lead to bleeding disorders such as combined coagulation factor V and VIII deficiency. The protein's dysfunction impairs the transport of coagulation factors disrupting their secretion. Additionally proteins like MCFD2 work closely with LMAN1 in this context as mutations in either protein can cause a similar deficiency highlighting the significant role of LMAN1 in maintaining normal hemostatic processes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
See full target information LMAN1
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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