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AB250640

重组Anti-LLGL1 + LLGL2抗体[EPR18899] - BSA and Azide free

Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LLGL1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

查看别名

DLG4, HUGL, HUGL1, LLGL1, Lethal(2) giant larvae protein homolog 1, LLGL, Hugl-1, Human homolog to the D-lgl gene protein

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18899]-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab183021 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling LLGL1 with ab183021 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18899]-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab183021 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunoprecipitation - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • IP

Supplier Data

Immunoprecipitation - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

LLGL1was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab183021 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab183021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : HepG2 whole cell lysate 10μg (Input).
Lane 2 : ab183021 IP in HepG2 whole cell lysate.
Lane 3 : Rabbit IgG, monoclonal [EPR18899] - Isotype Control (ab172730) instead of ab183021 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-LLGL1 + LLGL2 antibody [EPR18899] (<a href='/products/primary-antibodies/llgl1-llgl2-antibody-epr18899-ab183021'>ab183021</a>)

Predicted band size: 113 kDa

false

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • WB

Lab

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] (<a href='/products/primary-antibodies/llgl1-llgl2-antibody-epr18899-ab183021'>ab183021</a>) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

A459 cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 113 kDa

Observed band size: 115 kDa

false

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • WB

Supplier Data

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

Blocking buffer : 5% NFDM/TBST.

We recommend to use 1% SDS Hot lysis method to get desired WB results.

All lanes:

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] (<a href='/products/primary-antibodies/llgl1-llgl2-antibody-epr18899-ab183021'>ab183021</a>) at 1/2000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 15 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate prepared in 1% SDS Hot lysis method at 15 µg

Lane 3:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 15 µg

Lane 4:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared in 1% SDS Hot lysis method at 15 µg

Lane 5:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 15 µg

Lane 6:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared in 1% SDS Hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 113 kDa

Observed band size: 115 kDa

false

Exposure time: 20s

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • WB

Supplier Data

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

This data was developed using ab183021, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 3 minutes; Lane 3 : 15 seconds.

All lanes:

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] (<a href='/products/primary-antibodies/llgl1-llgl2-antibody-epr18899-ab183021'>ab183021</a>) at 1/2000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 113 kDa

Observed band size: 115 kDa

false

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)
  • WB

Supplier Data

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] - BSA and Azide free (AB250640)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LLGL1 + LLGL2 antibody [EPR18899] (<a href='/products/primary-antibodies/llgl1-llgl2-antibody-epr18899-ab183021'>ab183021</a>) at 1/10000 dilution

Lane 1:

His tagged human LLGL1 full length recombinant protein at 0.01 µg

Lane 2:

Flag tagged human LLGL2 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 113 kDa

Observed band size: 120 kDa

false

Exposure time: 40s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR18899

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen used for this product shares 53% identity (67% positives) with LLGL2. Cross-reactivity with this protein has not been confirmed experimentally.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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产品详情

ab250640 is the carrier-free version of ab183021.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The LLGL1 and LLGL2 proteins also known as Lethal (2) giant larvae proteins play important roles in cell polarity and asymmetric cell division. LLGL1 has a molecular mass of approximately 130 kDa and is expressed in various tissues with notable presence in the brain and reproductive organs. LLGL2 shares similar distribution and expression patterns. These proteins structurally contain several WD40 repeats involved in protein-protein interactions that help them in functioning as cortical factors.
Biological function summary

In epithelial cells and neural progenitors LLGL1 and LLGL2 are essential for maintaining cell polarity contributing to the structural stability and function of tissues. Both proteins are part of the Scribble complex a multi-protein assembly that regulates cell polarity and adhesion. By ensuring proper orientation and polarization of cells they are involved in tissue homeostasis and developmental processes.

Pathways

Cell polarity and division control involves the LLGL1 and LLGL2 proteins. They are key components of the PAR (Partitioning-defective) pathway interplaying with proteins like aPKC and PAR-6 essential for spatial cellular orientation. Additionally they play a role in the Hippo signaling pathway which is important for organ size regulation and cell proliferation working alongside proteins such as YAP/TAZ.

Dysfunction or alterations in LLGL1 and LLGL2 proteins have associations with cancer and neurological disorders. Loss of function can contribute to tumorigenesis highlighting their role as tumor suppressors in various cancer studies. In the context of neurological disorders improper regulation linked with these proteins can provide insight into brain development diseases interacting with proteins like Scribble and DLG variants.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.
See full target information LLGL1

其他靶点

LLGL2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 33:451-462 PubMed35775321

2022

Notoginsenoside R1 alleviates spinal cord injury by inhibiting oxidative stress, neuronal apoptosis, and inflammation via activating the nuclear factor erythroid 2 related factor 2/heme oxygenase-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Luo,Zhangli Bao,Mingjian Zhou,Yuxin Chen,Zhaoxi Huang
View all publications
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