Anti-LKB1 (phospho S428)抗体(ab63473)
Key features and details
- Rabbit polyclonal to LKB1 (phospho S428)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-LKB1 (phospho S428)抗体
参阅全部 LKB1 一抗 -
描述
兔多克隆抗体to LKB1 (phospho S428) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human LKB1 (phospho S428).
Database link: Q15831 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab63473 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63473于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
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| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
|
WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
靶标
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功能
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
组织特异性
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
疾病相关
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by a cAMP-dependent protein kinase. -
细胞定位
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
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数据库链接
- Entrez Gene: 20869 Mouse
- SwissProt: Q9WTK7 Mouse
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别名
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
see all
图片
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Western blot - Anti-LKB1 (phospho S428) antibody (ab63473)All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution
Lane 1 : mouse brain extracts
Lane 2 : mouse brain extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (ab63473)ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab63473 被引用在 10 文献中.
- Gao J et al. Wnt family member 1 (Wnt1) overexpression-induced M2 polarization of microglia alleviates inflammation-sensitized neonatal brain injuries. Bioengineered 13:12409-12420 (2022). PubMed: 35603707
- Zhu C et al. lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway. Bioengineered 13:13667-13679 (2022). PubMed: 35703318
- Zhang J et al. PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway. Cell Biol Int 45:305-319 (2021). PubMed: 33079432
- Wu J et al. Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation. Front Pharmacol 12:754976 (2021). PubMed: 34566665
- Kinsey CG et al. Protective autophagy elicited by RAF?MEK?ERK inhibition suggests a treatment strategy for RAS-driven cancers. Nat Med 25:620-627 (2019). PubMed: 30833748
- Hong B et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). PubMed: 29257268
- Ding Z et al. PCSK9 expression in the ischaemic heart and its relationship to infarct size, cardiac function, and development of autophagy. Cardiovasc Res 114:1738-1751 (2018). PubMed: 29800228
- Sueda T et al. BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells. Sci Rep 6:18949 (2016). PubMed: 26750638
- Yang SH et al. Lovastatin overcomes gefitinib resistance through TNF-a signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo. Oncotarget 6:23857-73 (2015). WB, IHC . PubMed: 26160843
- Konno H et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013). PubMed: 24119841