Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证

Anti-LKB1抗体[Ley 37D/G6] (ab15095)

Reviews (12)Q&A (9)References (39)
Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
  • Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

Key features and details

  • Mouse monoclonal [Ley 37D/G6] to LKB1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-LKB1抗体[Ley 37D/G6]
    参阅全部 LKB1 一抗

  • 描述

    小鼠单克隆抗体[Ley 37D/G6] to LKB1

  • 宿主

    Mouse

  • 特异性

    This antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1.

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant full length protein corresponding to Human LKB1.
    Database link: Q15831

  • 阳性对照

    • HAP1 and MEF cell lysates.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab15095于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (6)
1/100 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).
说明
WB
1/100 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).

靶标

  • 功能

    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.

  • 组织特异性

    Ubiquitously expressed. Strongest expression in testis and fetal liver.

  • 疾病相关

    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.

  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Phosphorylated by a cAMP-dependent protein kinase.

  • 细胞定位

    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt

  • 数据库链接

    • 别名

      • hLKB1 antibody
      • Liver kinase B1 antibody
      • LKB1 antibody
      • PJS antibody
      • Polarization related protein LKB1 antibody
      • Renal carcinoma antigen NY-REN-19 antibody
      • Serine/Threonine Kinase 11 antibody
      • Serine/threonine protein kinase 11 antibody
      • Serine/threonine protein kinase LKB1 antibody
      • Serine/threonine protein kinase STK11 antibody
      • Serine/threonine-protein kinase 11 antibody
      • Serine/threonine-protein kinase LKB1 antibody
      • Serine/threonine-protein kinase XEEK1 antibody
      • Stk11 antibody
      • STK11_HUMAN antibody
      see all

    图片

    • Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
      Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)

      Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
      ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
      Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)Image courtesy of anonymous abreviewer
      All lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution

      All lanes :

      Secondary
      All lanes : Polyclonal goat anti-mouse conjugated with horse radish peroxidase.

      Predicted band size: 48.6 kDa
      Observed band size: 55 kDa why is the actual band size different from the predicted?



      Negative control (Lane 1): LKB1 knockout MEF cells.

      Positive control (Lane 2): wildtype MEF cells.

      Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.

      Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.

       

      See Abreview

    文献 (39)

    发表研究结果有使用 ab15095？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab15095 被引用在 39 文献中.

    • Pencik J  et al. STAT3/LKB1 controls metastatic prostate cancer by regulating mTORC1/CREB pathway. Mol Cancer 22:133 (2023). PubMed: 37573301
    • Li J  et al. Temperature Elevation during Semen Delivery Deteriorates Boar Sperm Quality by Promoting Apoptosis. Animals (Basel) 13:N/A (2023). PubMed: 37893927
    • Gao Y  et al. Inactivation of AMPK Leads to Attenuation of Antigen Presentation and Immune Evasion in Lung Adenocarcinoma. Clin Cancer Res 28:227-237 (2022). PubMed: 34667030
    • Ming Y  et al. Interaction of Nuclear Receptor Subfamily 4 Group A Member 1 (Nr4a1) and Liver Linase B1 (LKB1) Mitigates Type 2 Diabetes Mellitus by Activating Monophosphate-Activated Protein Kinase (AMPK)/Sirtuin 1 (SIRT1) Axis and Inhibiting Nuclear Factor-kappa B (NF-?B) Activation. Med Sci Monit 26:e920278 (2020). PubMed: 31939452
    • Zhao F  et al. Scleral HIF-1a is a prominent regulatory candidate for genetic and environmental interactions in human myopia pathogenesis. EBioMedicine 57:102878 (2020). PubMed: 32652319
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 21 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung adenocarcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 8.5 buffer
    Permeabilization
    No
    Specification
    Lung adenocarcinoma
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Allan Wang

    Verified customer

    提交于 Mar 20 2018

    Western blot abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris nupage gel)
    Loading amount
    10 µg
    Specification
    A549
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 12 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hepatocarcinoma (HCC))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6, 20 min at 97ºC IN A PT LINK
    Permeabilization
    No
    Specification
    Hepatocarcinoma (HCC)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Virginia Gutiérrez

    Verified customer

    提交于 Jan 07 2016

    Western blot abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 10 2013

    Western blot abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neurons)
    Loading amount
    50 µg
    Specification
    neurons
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 31 2011

    Question

    I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium

    I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.

    I guess it might be possible that at Trial (testing) discount might be applicable?

    Read More
    Answer

    Thank you for contacting us.

    I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have contacted the lab and indeed all the immunogens of the anti-LKB1antibodies guaranteed to work in WB/IF in our catalogue have a 28-50% sequence homology with the Dictyostelium protein and therefore are not likely to work.

    The amino acid sequence for LKB1 for Dictyostelium that I used can be found at http://www.uniprot.org/uniprot/Q15831.fasta. Unfortunately, this is the predicted amino acid sequence for the species and has not been confirmed therefore, if your customer has the verified sequence of this protein, then I will be happy to repeat these alignments to verify the homology.

    Regarding an anti-CAMKK antibody, I am not able to recommend a suitable one unless I have the Dictyostelium amino acid sequence for this protein. If your customer could send it, then again I will be happy to perform the sequence alignments.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium

    I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.

    I guess it might be possible that at Trial (testing) discount might be applicable?

    I look forward to your feedback which I can forward to the customer.

    Read More
    Answer

    Thank you for contacting us.

    I have found the amino acid sequence for LKB1 for Dictyostelium which can be found at http://www.uniprot.org/uniprot/Q15831.fasta

    I have contacted the lab to see if any of our antibodies would be compatible for use in Dictyostelium.

    I have not been able to find the sequence for CAMKK for Dictyostelium. Could you ask your customer to send it if they have it so that I may perform the sequence alignments?

    I look forward to your reply.

    Read More

    Question

    What is the epitope for antibody ab15095?

    Read More
    Answer

    Thank you for contacting us.

    I had a quick chat with my colleague in lab. They have confirmed that the antibody has been raised against the full length protein so they do not have determined the epitope this antibody is recognizing

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    I wonder if you have the immunizing peptide for ab15095 (anti-Lkb1) for sale. I am planning to use the antibody for IHC-P and would like to include a negative control with a neutralized antibody to exclude false positive staining/non-specific binding. Thanks in advance for your help.

    Read More
    Answer

    Thank you for contacting us.

    I have checked with supplying the lab, but unfortunately, the immunogen is not available for sale.

    Alternatively, I could recommend an isotype control (mouse IgG2b) to be used as negative control, for instance:

    ab91366 (Mouse IgG2b [PLPV219] - Isotype Control)
    https://www.abcam.com/Mouse-IgG2b-PLPV219-Isotype-Control-ab91366.html

    ab18421 (Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control)
    https://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MG2b-57-Isotype-control-ab18421.html

    ab18469 (Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control)
    https://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MPC-11-Isotype-control-ab18469.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    I wonder if you have the immunizing peptide for ab15095 (anti-Lkb1) for sale. I am planning to use the antibody for IHC-P and would like to include a negative control with a neutralized antibody to exclude false positive staining/non-specific binding. Thanks in advance for your help.

    Read More
    Answer

    Thank you for contacting us.

    The immunogen was ahighly purified recombinant full length protein made in E. coli, but is not a peptide. I will check with the lab if the immunogen might be commercially available and let you know what I find out.

    Thank you for your patience and understanding.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    1-10 of 21 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com