Anti-liver FABP抗体[L2B10] (ab7366)
![Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.cn/ps/products/7/ab7366/Images/ab7366-282298-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)")
Key features and details
- Mouse monoclonal [L2B10] to liver FABP
- Suitable for: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG1
概述
-
产品名称
Anti-liver FABP抗体[L2B10]
参阅全部 liver FABP 一抗 -
描述
小鼠单克隆抗体[L2B10] to liver FABP -
宿主
Mouse -
特异性
This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP. -
经测试应用
适用于: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Frmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Cow, Human, African green monkey -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
L2B10 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054
|
| Functional Studies |
Use at an assay dependent concentration.
Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant. |
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054 |
|
Functional Studies
Use at an assay dependent concentration. Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant. |
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
靶标
-
功能
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
结构域
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 2168 Human
- Entrez Gene: 14080 Mouse
- Entrez Gene: 24360 Rat
- Omim: 134650 Human
- SwissProt: P07148 Human
- SwissProt: P12710 Mouse
- SwissProt: P02692 Rat
- Unigene: 380135 Human
see all -
别名
- FABP 1 antibody
- FABP1 antibody
- FABPL antibody
see all
图片
-
Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.cn/ps/products/7/ab7366/Images/ab7366-222193-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.cn/ps/products/7/ab7366/Images/ab7366-222192-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.
-
![Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.cn/ps/products/7/ab7366/Images/ab7366-12927-anti-liver-fabp-antibody-l2b10-flow-cytometry.jpg)
Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (18)
ab7366 被引用在 18 文献中.
- Tajima O et al. Ganglioside GM3 prevents high fat diet-induced hepatosteatosis via attenuated insulin signaling pathway. PLoS One 18:e0281414 (2023). PubMed: 36827398
- Niture S et al. MicroRNA-483-5p Inhibits Hepatocellular Carcinoma Cell Proliferation, Cell Steatosis, and Fibrosis by Targeting PPARα and TIMP2. Cancers (Basel) 15:N/A (2023). PubMed: 36980601
- Niture S et al. Cyanotoxins Increase Cytotoxicity and Promote Nonalcoholic Fatty Liver Disease Progression by Enhancing Cell Steatosis. Toxins (Basel) 15:N/A (2023). PubMed: 37505679
- Uhlitz F et al. Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer. EMBO Mol Med 13:e14123 (2021). PubMed: 34409732
- Belcastro L et al. Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity. Nutrients 13:N/A (2021). PubMed: 34444927