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Anti-Lipoprotein lipase抗体[LPL.A4] (ab21356)

Reviews (9)Q&A (11)References (61)
Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
  • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)

Key features and details

  • Mouse monoclonal [LPL.A4] to Lipoprotein lipase
  • Suitable for: Flow Cyt, WB
  • Reacts with: Cow, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Lipoprotein lipase抗体[LPL.A4]
    参阅全部 Lipoprotein lipase 一抗

  • 描述

    小鼠单克隆抗体[LPL.A4] to Lipoprotein lipase

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, WBmore details

  • 种属反应性

    与反应: Cow, Human

  • 免疫原

    Full length protein corresponding to Human Lipoprotein lipase.

  • 阳性对照

    • Purified human and bovine recombinant Lipoprotein lipase.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab21356于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (4)
1/1000. Predicted molecular weight: 53 kDa.
说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 53 kDa.

靶标

  • 功能

    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.

  • 疾病相关

    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.

  • 序列相似性

    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.

  • 翻译后修饰

    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

  • 细胞定位

    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Information by UniProt

  • 数据库链接

    • 别名

      • EC 3.1.1 antibody
      • EC 3.1.1.34 antibody
      • HDLCQ11 antibody
      • LIPD antibody
      • LIPL_HUMAN antibody
      • Lipoprotein lipase antibody
      • LPL antibody
      • LPL protein antibody
      • MGC137861 antibody
      see all

    图片

    • Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      All lanes : Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

      Lane 1 : Human Lipoprotein lipase.
      Lane 2 : Bovine Lipoprotein lipase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 53 kDa
      Observed band size: 55,60 kDa why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356)
      Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献 (61)

    发表研究结果有使用 ab21356？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab21356 被引用在 61 文献中.

    • Li H  et al. Geniposide plus chlorogenic acid reverses non-alcoholic steatohepatitis via regulation of gut microbiota and bile acid signaling in a mouse model in vivo. Front Pharmacol 14:1148737 (2023). PubMed: 37077819
    • Tang W  et al. Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease. Hum Mol Genet 32:1466-1482 (2023). PubMed: 36519761
    • Chen L  et al. Attenuation of Alzheimer's brain pathology in 5XFAD mice by PTH1-34, a peptide of parathyroid hormone. Alzheimers Res Ther 15:53 (2023). PubMed: 36918976
    • Ding S  et al. Deficiency of angiopoietin-like 4 enhances CD8+ T cell bioactivity via metabolic reprogramming for impairing tumour progression. Immunology 170:28-46 (2023). PubMed: 37094816
    • Ozaki E  et al. Minocycline suppresses disease-associated microglia (DAM) in a model of photoreceptor cell degeneration. Exp Eye Res 217:108953 (2022). PubMed: 35090890
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 20 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: HistoVT
    Permeabilization
    Yes - 0.1% Triton-X 100
    Specification
    brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    雄介 岸

    Verified customer

    提交于 Sep 15 2023

    Immunohistochemistry (Frozen sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    Roche Blocking Reagent as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 11 2021

    Immunohistochemistry free floating abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain (PFA fixed))
    Specification
    Brain (PFA fixed)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 05 2020

    Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Sample
    Mouse Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 11 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1.5µg/mL · Temperature: 23°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citra Plus
    Sample
    Mouse Tissue sections (Small Intestine)
    Specification
    Small Intestine
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Aug 28 2013

    Western blot abreview for Anti-Lipoprotein lipase antibody [LPL.A4]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    30 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Dongil Kim

    Verified customer

    提交于 Apr 11 2013

    Question

    Can you tell me if the epitope of this Ab is located in an extracellular, cytoplasmatic or transmembrane region of the LPL protein.

    Read More
    Answer

    This antibody was raised against the whole Human LPL whole protein as stated in the datasheet.

    Unfortunately, the exact epitope is unknown as the whole protein with all regions intact was used to produce the antibody and the epitope has not been mapped.

    Read More

    Question

    Yes, that’s my order. A credit would be greatly appreciated. The conc we used was 1:10,000.

    Thanks

    Read More
    Answer

    Thank you for your reply and for confirming those details.

    Your credit note ID is ***.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting departmentat us.credits@abcam.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department.

    Please let me know if there is anything else that we can do for you and I'll be happy to help. Thank you again for your patience in resolving these issues.

    Read More

    Question

    Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

    The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

    Thanks so much for your help.

    Read More
    Answer

    One more thing. Could you please tell me what dilution you've tried with this secondary antibody, for our records?

    Thank you so much!

    Read More

    Question

    Your QC data looks really good. I think the antibody may be unstable to the current shipping methods? But anyway, we will just going to use it at high concentration for all future exp.

    The secondary antibody that did not work is “https://srm.shire.com/scripts/wgate/bbpsc04/˜flNlc3Npb249U1NQOnNwZ3BkczAxMTowMDAwLjAwMzEuMTk5YjhiMzUuOGJkNiZ-aHR0cF9jb250ZW50X2NoYXJzZXQ9aXNvLTg4NTktMSZ-U3RhdGU9MjM3NTMuMDI2LjAyLjAy?gv_selected_line=19&˜okcode=SEL_YOUR_DISPLAY”. It also did not work with a mouse anti-His primary antibody.

    Thanks so much for your help.

    Read More
    Answer

    Thank you for your reply.

    I have recorded all of the information so that we can further investigate the trouble with the antibody. I again apologize that it is not working as expecting, but I'm glad you've found a way to get some results.

    With the ab98693, I've found an order on the PO#***. Can you confirm that this is your order? I will process the credit or refund, whichever you prefer.

    I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

    1-10 of 20 Abreviews or Q&A

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