AB21356

Anti-Lipoprotein lipase 抗体 [LPL.A4]

Name: Lipoprotein lipase抗体[LPL.A4] (ab21356)| Abcam中文官网
Brand: Abcam Limited
SKU: AB21356
Price: 5083 CNY
Availability: InStock
Rating: 4 (8 reviews)

Anti-Lipoprotein lipase antibody [LPL.A4]

(8 Reviews)

(64 Publications)

Mouse Monoclonal Lipoprotein lipase antibody. Suitable for Flow Cyt, WB and reacts with Human, Cow samples. Cited in 64 publications. Immunogen corresponding to Full Length Protein corresponding to Human LPL.

查看别名

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

2 Images

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Flow Cyt

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Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

All lanes:

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

Lane 1:

Human Lipoprotein lipase. at 10 µg

Lane 2:

Bovine Lipoprotein lipase at 10 µg

Predicted band size: 53 kDa

Observed band size: 55 kDa,60 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

LPL.A4

亚型

IgG1

不含载体蛋白

反应种属

Cow, Human

应用

Flow Cyt, WB

applications

免疫原

Full Length Protein corresponding to Human LPL. The exact immunogen used to generate this antibody is proprietary information.

P06858

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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产品详情

This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Protein A affinity chromatography

存储溶液

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

See full target information LPL

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文献 (64)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400064 PubMed38981007

2024

Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Chan Park,Jong Won Han,Woochan Lee,Jieun Kim,Sang-Eun Lee,Dongjoon Lee,Hayoung Choi,Jihui Han,You Jung Kang,Yen N Diep,Hansang Cho,Rian Kang,Won Jong Yu,Jean Lee,Murim Choi,Sun-Wha Im,Jong-Il Kim,Inhee Mook-Jung

Immunology 170:28-46 PubMed37094816

2023

Deficiency of angiopoietin-like 4 enhances CD8 T cell bioactivity via metabolic reprogramming for impairing tumour progression.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Ding,Zhijie Lin,Xiaoyuan Zhang,Xiaoqing Jia,Hualing Li,Yi Fu,Xuefeng Wang,Guoqiang Zhu,Guotao Lu,Weiming Xiao,Weijuan Gong

Frontiers in pharmacology 14:1148737 PubMed37077819

2023

Geniposide plus chlorogenic acid reverses non-alcoholic steatohepatitis regulation of gut microbiota and bile acid signaling in a mouse model .

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Xin Xin,Qin Feng,Yiyang Hu

Alzheimer's research & therapy 15:53 PubMed36918976

2023

Attenuation of Alzheimer's brain pathology in 5XFAD mice by PTH, a peptide of parathyroid hormone.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Lei Xiong,Lingling Yao,Jinxiu Pan,Emily Arzola,Xiaojuan Zhu,Lin Mei,Wen-Cheng Xiong

Molecular neurodegeneration 17:83 PubMed36536457

2022

Amyloid-beta and tau pathologies act synergistically to induce novel disease stage-specific microglia subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Won Kim,Kevin J Tu,Alice Wei,Ashley J Lau,Anabel Gonzalez-Gil,Tianyu Cao,Kerstin Braunstein,Jonathan P Ling,Juan C Troncoso,Philip C Wong,Seth Blackshaw,Ronald L Schnaar,Tong Li

Human molecular genetics 32:1466-1482 PubMed36519761

2022

Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Willcyn Tang,John Thundyil,Grace Gui Yin Lim,Teddy J W Tng,Sean Qing Zhang Yeow,Aditya Nair,Chou Chai,Tso-Pang Yao,Kah-Leong Lim

Frontiers in nutrition 9:914079 PubMed36091227

2022

Gypenosides ameliorate high-fat diet-induced non-alcoholic steatohepatitis farnesoid X receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshan Li,Yingfei Xi,Hongliang Liu,Xin Xin

Frontiers in pharmacology 13:848957 PubMed35652039

2022

Astilbin Activates the Reactive Oxidative Species/PPARγ Pathway to Suppress Effector CD4 T Cell Activities Direct Binding With Cytochrome P450 1B1.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Ding,Guotao Lu,Biying Wang,Jie Xiang,Chunxia Hu,Zhijie Lin,Yanbing Ding,Weiming Xiao,Weijuan Gong

Journal of neuroinflammation 19:61 PubMed35236374

2022

Microglial VPS35 deficiency impairs Aβ phagocytosis and Aβ-induced disease-associated microglia, and enhances Aβ associated pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ren,Lingling Yao,YongGang Wang,Lin Mei,Wen-Cheng Xiong

Nature communications 13:945 PubMed35177618

2022

Stroke induces disease-specific myeloid cells in the brain parenchyma and pia.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Beuker,David Schafflick,Jan-Kolja Strecker,Michael Heming,Xiaolin Li,Jolien Wolbert,Antje Schmidt-Pogoda,Christian Thomas,Tanja Kuhlmann,Irene Aranda-Pardos,Noelia A-Gonzalez,Praveen Ashok Kumar,Yves Werner,Ertugrul Kilic,Dirk M Hermann,Heinz Wiendl,Ralf Stumm,Gerd Meyer Zu Hörste,Jens Minnerup

View all publications

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Anti-Lipoprotein lipase 抗体 [LPL.A4]

Anti-Lipoprotein lipase antibody [LPL.A4]

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

Western blot - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原