重组Anti-Lipoprotein a抗体[EPR6474] (ab125014)
![Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)](https://www.abcam.cn/ps/products/125/ab125014/Images/ab125014-253607-anti-lipoprotein-a-antibody-epr6474-western-blot.jpg "Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6474] to Lipoprotein a
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lipoprotein a抗体[EPR6474]
参阅全部 Lipoprotein a 一抗 -
描述
兔单克隆抗体[EPR6474] to Lipoprotein a -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Lipoprotein a aa 150-250. The exact sequence is proprietary.
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阳性对照
- WB: Human plasma lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6474 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125014于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 501 kDa (predicted molecular weight: 501 kDa).
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 501 kDa (predicted molecular weight: 501 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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相关性
Lipoprotein(a) (Lp(a)) is a lipoprotein subclass assembled in the blood from low density lipoprotein (LDL) molecules and apolipoprotein-a (apo-a). Lp(a) recruits inflammatory cells through interaction with Mac-1 integrin. High Lp(a) in blood is a risk factor for coronary heart disease, cerebrovascular disease, atherosclerosis, thrombosis, and stroke. Lp(a) concentrations may be affected by disease states, but are only moderately affected by diet, exercise and other environmental factors. Lipid-reducing drugs have no effect on Lp(a) concentration. High Lp(a) predicts risk of early atherosclerosis similar to high LDL, but in advanced atherosclerosis, Lp(a) is a risk factor independent of LDL, indicating a coagulant risk of plaque thrombosis. Apo(a) contains domains that are very similar to plasminogen (PLG). Lp(a) accumulates in the vessel wall and inhibits binding of PLG to the cell surface, reducing plasmin generation which increases clotting. This inhibition also promotes proliferation of smooth muscle cells. These unique features of Lp(a) suggest a role in the generation of clots and atherosclerosis. -
数据库链接
- Entrez Gene: 4018 Human
- Omim: 152200 Human
- SwissProt: P08519 Human
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别名
- AK38 antibody
- Antiangiogenic AK38 protein antibody
- apo(a) antibody
see all
图片
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Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/50000 dilution (purified) + Human plasma lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 501 kDa
Observed band size: 501 kDaBlocking and dilution buffer: 5% NFDM /TBST.
The observed bands are consistant with desciption in PMID:10703674, which should be N-terminal fragments.
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![Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)](https://www.abcam.cn/ps/products/125/ab125014/Images/ab125014-139132-anti-lipoprotein-a-antibody-epr6474-western-blot.jpg)
Western blot - Anti-Lipoprotein a antibody [EPR6474] (ab125014)Anti-Lipoprotein a antibody [EPR6474] (ab125014) at 1/20000 dilution (unpurified) + Human plasma lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 501 kDa
Observed band size: 500 kDa why is the actual band size different from the predicted? -
![Anti-Lipoprotein a antibody [EPR6474] (ab125014)](https://www.abcam.cn/ps/products/125/ab125014/Images/ab125014-5-benefits-of-recombinant-antibodies.png)
Anti-Lipoprotein a antibody [EPR6474] (ab125014)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab125014 被引用在 4 文献中.
- Lin YK et al. Apolipoprotein (a)/Lipoprotein(a)-Induced Oxidative-Inflammatory a7-nAChR/p38 MAPK/IL-6/RhoA-GTP Signaling Axis and M1 Macrophage Polarization Modulate Inflammation-Associated Development of Coronary Artery Spasm. Oxid Med Cell Longev 2022:9964689 (2022). PubMed: 35096275
- Oliveira C et al. Apolipoprotein(a) inhibits hepatitis C virus entry through interaction with infectious particles. Hepatology 65:1851-1864 (2017). PubMed: 28152568
- Raal FJ et al. PCSK9 inhibition-mediated reduction in Lp(a) with evolocumab: an analysis of 10 clinical trials and the LDL receptor's role. J Lipid Res 57:1086-96 (2016). PubMed: 27102113
- Müller N et al. IL-6 blockade by monoclonal antibodies inhibits apolipoprotein (a) expression and lipoprotein (a) synthesis in humans. J Lipid Res 56:1034-42 (2015). PubMed: 25713100