Lipocalin-2 / NGAL重组抗体[RM1211]_Lipocalin-2 / NGAL抗体(ab318210)

产品名称

Anti-Lipocalin-2 / NGAL抗体[RM1211] - BSA and Azide free
参阅全部 Lipocalin-2 / NGAL 一抗
描述

兔重组multiclonal [RM1211] to Lipocalin-2 / NGAL - BSA and Azide free
宿主

Rabbit
特异性

unsuitable for rat WB
经测试应用

适用于: WB, IHC-Pmore details
不适用于: Flow Cyt (Intra),ICC/IF or IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: SW480, COLO 205, A549, Human bone marrow, Human lung, Mouse bone marrow, Mouse spleen lysates. IHC-P: Human spleen, Human placenta, human cerebrum, human cardiac muscle, Mouse spleen, Mouse lung, Mouse pancreatic tumor, Rat spleen, Rat lung tissues.
常规说明
ab318210 is the carrier-free version of ab318209
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C.
存储溶液

pH: 7.20
Constituent: 100% PBS
无载体

是
Concentration information loading...
纯度

Protein A purified
克隆

Recombinant Multiclonal
克隆编号

RM1211
同种型

IgG
研究领域

Alternative Versions
- Anti-Lipocalin-2 / NGAL antibody [RM1211] (ab318209)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

The Abpromise guarantee

Abpromise™承诺保证使用ab318210于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

说明
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

应用说明

Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

功能

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
组织特异性

Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
序列相似性

Belongs to the calycin superfamily. Lipocalin family.
细胞定位

Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
Target information above from: UniProt accession P80188 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3934 Human
- Entrez Gene: 16819 Mouse
- Entrez Gene: 170496 Rat
- Omim: 600181 Human
- SwissProt: P80188 Human
- SwissProt: P11672 Mouse
- SwissProt: P30152 Rat
- Unigene: 204238 Human
- Unigene: 9537 Mouse
- Unigene: 11303 Rat
see all
别名
- 24p3 antibody
- 25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
- Alpha 2 microglobulin related protein antibody
- HGNC:6526 antibody
- HNL antibody
- Lcn 2 antibody
- Lcn2 antibody
- Lipocalin-2 antibody
- Migration stimulating factor inhibitor antibody
- MSFI antibody
- Neutrophil gelatinase associated lipocalin antibody
- Neutrophil gelatinase associated lipocalin precursor antibody
- Neutrophil gelatinase-associated lipocalin antibody
- NGAL antibody
- NGAL_HUMAN antibody
- Oncogene 24p3 antibody
- Oncogenic lipocalin 24p3 antibody
- p25 antibody
- Siderocalin antibody
- siderocalin LCN2 antibody
- SV40 induced 24P3 protein antibody
- Uterocalin antibody
see all

Western blot - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

All lanes : Anti-Lipocalin-2 / NGAL antibody [RM1211] (ab318209) at 1/1000 dilution

Lane 1 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : COLO 205 (human colon adenocarcinoma tumor ascitic epithelial cell) whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

This data was developed using ab318209, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: 293T.

Low expression: A549 (PMID: 32712458)

The identity of the higher MW band at approximately 100 kDa (in lane 2, 3) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

All lanes : Anti-Lipocalin-2 / NGAL antibody [RM1211] (ab318209) at 1/1000 dilution

Lane 1 : Human bone marrow tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Mouse bone marrow tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?

This data was developed using ab318209, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The identity of the higher MW band at approximately 100 kDa in recombinant multiclonal ab318209 (in lane 2) is unknown.

Anti-Lipocalin-2 / NGAL antibody (ab318209) staining at 1/1000 dilution can work on human species, which is better than Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1/1000 dilution.

Exposure time: Recombinant multiclonal ab318209: Lane 1: 1 seconds; Lane 2: 6 seconds, Lane 3: 1 second Polyclonal ab63939: 15 seconds
Western blot - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

All lanes : Anti-Lipocalin-2 / NGAL antibody [RM1211] (ab318209) at 1/1000 dilution

Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?

This data was developed using ab318209, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression tissue: heart (PMID: 28615213), brain (PMID: 20860895, PMID: 21184133)

Anti-Lipocalin-2 / NGAL antibody (ab318209) staining at 1/1000 dilution showed better than Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1/1000 dilution.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Recombinant multiclonal ab318209: 10 seconds Polyclonal ab63939: 103 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human spleen. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human placenta. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of mouse lung. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic tumor tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse pancreatic tumor. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of rat lung. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: scattered staining on immune cells of human cerebrum. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: scattered staining on immune cells of human cardiac muscle. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on mouse cerebrum. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on mouse cardiac muscle. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on rat cerebrum. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

This data was developed using ab318209, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Lipocalin-2 / NGAL with ab318209 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on rat cardiac muscle. The section was incubated with ab318209 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Anti-Lipocalin-2 / NGAL antibody [RM1211] - BSA and Azide free (ab318210)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

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概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

无载体

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

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