重组Anti-Lipin 2/LPIN2抗体[EPR12479] (ab176347)
![Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)](https://www.abcam.cn/ps/products/176/ab176347/Images/ab176347-203775-anti-lipin-2-lpin2-antibody-epr12479-western-blot.jpg "Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12479] to Lipin 2/LPIN2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lipin 2/LPIN2抗体[EPR12479]
参阅全部 Lipin 2/LPIN2 一抗 -
描述
兔单克隆抗体[EPR12479] to Lipin 2/LPIN2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human Lipin 2/LPIN2 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q92539 -
阳性对照
- WB: Jurkat, HepG2, 293T and HeLa whole cell lysate (ab150035). Flow Cyt (intra): Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12479 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176347于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism. -
组织特异性
Expressed in liver, lung, kidney, placenta, spleen, thymus, lymph node, prostate, testes, small intestine, and colon. -
疾病相关
Defects in LPIN2 are the cause of Majeed syndrome (MAJEEDS) [MIM:609628]. An autosomal recessive syndrome characterized by chronic recurrent multifocal osteomyelitis that is of early onset with a lifelong course, congenital dyserythropoietic anemia that presents as hypochromic, microcytic anemia during the first year of life and ranges from mild to transfusion-dependent, and transient inflammatory dermatosis, often manifesting as Sweet syndrome (neutrophilic skin infiltration). -
序列相似性
Belongs to the lipin family. -
结构域
Contains 1 Asp-Xaa-Asp-Xaa-Thr (DXDXT) motif, a catalytic motif known to be essential for phosphatidate phosphatase activity.
Contains one Leu-Xaa-Xaa-Ile-Leu (LXXIL) motif, a motif known to be a transcriptional binding motif. -
细胞定位
Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum membrane. Translocates to endoplasmic reticulum membrane with increasing levels of oleate. - Information by UniProt
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数据库链接
- Entrez Gene: 9663 Human
- Entrez Gene: 64898 Mouse
- Entrez Gene: 316737 Rat
- Omim: 605519 Human
- SwissProt: Q92539 Human
- SwissProt: Q99PI5 Mouse
- Unigene: 132342 Human
- Unigene: 227924 Mouse
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别名
- KIAA0249 antibody
- Lipin-2 antibody
- Lipin2 antibody
see all
图片
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Western blot - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)All lanes : Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
![Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)](https://www.abcam.cn/ps/products/176/ab176347/Images/ab176347-203777-anti-lipin-2-lpin2-antibody-epr12479-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Lipin 2/LPIN2 with ab176347 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)](https://www.abcam.cn/ps/products/176/ab176347/Images/ab176347-3-benefits-of-recombinant-antibodies.png)
Anti-Lipin 2/LPIN2 antibody [EPR12479] (ab176347)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab176347 被引用在 5 文献中.
- Zhang X et al. Lipin2 ameliorates diabetic encephalopathy via suppressing JNK/ERK-mediated NLRP3 inflammasome overactivation. Int Immunopharmacol 118:109930 (2023). PubMed: 37001383
- Dou JY et al. Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation. Biomol Ther (Seoul) 30:246-256 (2022). PubMed: 34815367
- Cui ZY et al. Allium victorialis L. Extracts Promote Activity of FXR to Ameliorate Alcoholic Liver Disease: Targeting Liver Lipid Deposition and Inflammation. Front Pharmacol 12:738689 (2021). PubMed: 34690775
- Baneux C et al. Phosphatidic acid metabolism regulates neuroendocrine secretion but is not under the direct control of lipins. IUBMB Life 72:533-543 (2020). PubMed: 31967386
- Zhao Y et al. Gain-of-Function Mutations of SLC16A11 Contribute to the Pathogenesis of Type 2 Diabetes. Cell Rep 26:884-892.e4 (2019). PubMed: 30673611