Anti-Lipid A抗体[26-5] (ab8467)
Key features and details
- Mouse monoclonal [26-5] to Lipid A
- Suitable for: Agglutination, ELISA
- Reacts with: Species independent
- Isotype: IgG2b
概述
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产品名称
Anti-Lipid A抗体[26-5] -
描述
小鼠单克隆抗体[26-5] to Lipid A -
宿主
Mouse -
经测试应用
适用于: Agglutination, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Other Immunogen Type corresponding to Lipid A. Synthetic Lipid A.
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常规说明
Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 99.88% PBS -
Concentration information loading... -
纯度
IgG fraction -
纯化说明
This product is sterile filtered. -
Primary antibody说明
Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria. -
克隆
单克隆 -
克隆编号
26-5 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Agglutination |
Use at an assay dependent concentration.
Use NEAT. |
|
| ELISA |
1/10.
in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density. |
| 说明 |
|---|
|
Agglutination
Use at an assay dependent concentration. Use NEAT. |
|
ELISA
1/10. in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density. |
靶标
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相关性
Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction. -
细胞定位
Secreted
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab8467 被引用在 8 文献中.
- Yang Y et al. A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain. Front Aging Neurosci 15:1126273 (2023). PubMed: 37020861
- Yang Y et al. Palmitate lipotoxicity is closely associated with the fatty acid-albumin complexes in BV-2 microglia. PLoS One 18:e0281189 (2023). PubMed: 37079515
- Chen PP et al. Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease. Theranostics 13:3988-4003 (2023). PubMed: 37554279
- Kameli N et al. Characterization of Feces-Derived Bacterial Membrane Vesicles and the Impact of Their Origin on the Inflammatory Response. Front Cell Infect Microbiol 11:667987 (2021). PubMed: 34026664
- Schlichtner S et al. Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis. Int Immunopharmacol 100:108155 (2021). PubMed: 34543981
- Vargas-Caraveo A et al. Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions. Sci Rep 7:13113 (2017). PubMed: 29030613
- Martin I et al. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol 194:3924-36 (2015). PubMed: 25780044
- Kadowaki T et al. Functional Characterization of Lipopolysaccharide derived from Symbiotic Bacteria in Rice as a Macrophage-activating Substance. Anticancer Res 31:2467-76 (2011). WB . PubMed: 21873161