重组Anti-Lingo1抗体[EPR24049-72] - BSA and Azide free

• WB

Lab

Western blot - Anti-Lingo1 antibody [EPR24049-72] - BSA and Azide free (AB287989)

This data was developed using ab309529, the same antibody clone in a different buffer formulation.

Lanes 1 - 9 : Merged signal (magenta and green). Green – ab309529 observed at 93 kDa. Magenta - loading control, ab8245 observed at 37 kDa.

ab309529 was shown to specifically react with LINGO1 in human, mouse and rat brain tissue lysates. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. ab287974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lingo1 antibody [EPR24049-72] (<a href='/products/primary-antibodies/lingo1-antibody-epr24049-72-ab309529'>ab309529</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 2:

Mouse brain tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 3:

Rat brain tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 4:

Human kidney tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 5:

Mouse kidney tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 6:

Rat kidney tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 7:

Human skeletal muscle tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 8:

Mouse skeletal muscle tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 9:

Rat skeletal muscle tissue lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Secondary

Lanes 1 - 9:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) at 1/20000 dilution

Lanes 1 - 9:

Goat anti-Mouse IgG H&L (IRDye® 680RD) at 1/20000 dilution

Observed band size: 93 kDa,37 kDa

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