Anti-LINE-1 ORF2p抗体(ab106004)
Key features and details
- Chicken polyclonal to LINE-1 ORF2p
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgY
概述
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产品名称
Anti-LINE-1 ORF2p抗体
参阅全部 LINE-1 ORF2p 一抗 -
描述
鸡多克隆抗体to LINE-1 ORF2p -
宿主
Chicken -
特异性
L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. Reactivity occurs against human L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
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经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee
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免疫原
Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein.
Database link: O00370 -
阳性对照
- induced bacterially expressed human ORF2
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常规说明
Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening product.ab106004 is intended for use in studying control of L1 retrotransposons.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯化说明
L1/ORF2 Antibody is directed against two regions within the endonuclease domain of L1 ORF2 protein. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106004于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 149 kDa. |
靶标
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数据库链接
- SwissProt: O00370 Human
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别名
- L1/ORF2 antibody
- LINE 1 ORF2p antibody
- LINE-1 retrotransposable element ORF2 protein antibody
see all
图片
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Western blot - Anti-LINE-1 ORF2p antibody (ab106004)All lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution
Lane 1 : induced bacterially expressed human ORF2
Lane 2 : uninduced bacterially expressed human ORF2
Predicted band size: 149 kDaNote: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human LINE-1 ORF2p endonuclease domain fusion protein.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab106004 被引用在 4 文献中.
- Zhang H et al. Single-nucleus transcriptomic landscape of primate hippocampal aging. Protein Cell 12:695-716 (2021). PubMed: 34052996
- Bi S et al. SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer. Protein Cell 11:483-504 (2020). PubMed: 32504224
- Romero MA et al. Acute and chronic resistance training downregulates select LINE-1 retrotransposon activity markers in human skeletal muscle. Am J Physiol Cell Physiol 314:C379-C388 (2018). PubMed: 29351416
- Thomas CA et al. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation. Cell Stem Cell 21:319-331.e8 (2017). PubMed: 28803918