Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Lin28A抗体[EPR4640] - BSA and Azide free (ab226089)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
  • Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
  • Immunoprecipitation - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
  • Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4640] to Lin28A - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

概述

  • 产品名称

    Anti-Lin28A抗体[EPR4640] - BSA and Azide free
    参阅全部 Lin28A 一抗

  • 描述

    兔单克隆抗体[EPR4640] to Lin28A - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IP, ICC/IF, WBmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab226089 is the carrier-free version of ab124765.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.2
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4640

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226089于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

  • 组织特异性

    Expressed in embryonic stem cells (ES cells), placenta and testis.

  • 序列相似性

    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.

  • 发展阶段

    Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.

  • 结构域

    The CSD domain is required for function in muscle differentiation.

  • 细胞定位

    Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CSDD1 antibody
      • FLJ12457 antibody
      • LIN 28 antibody
      • Lin 28 homolog A (C. elegans) antibody
      • Lin-28A antibody
      • LIN28 antibody
      • LIN28A antibody
      • LN28A_HUMAN antibody
      • Protein lin-28 homolog A antibody
      • ZCCHC1 antibody
      • zinc finger CCHC domain containing 1 antibody
      • Zinc finger CCHC domain-containing protein 1 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
      Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

      Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/500 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    • Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
      Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

      Intracellular Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/60 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765). 

       

       

    • Immunoprecipitation - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
      Immunoprecipitation - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

      ab124765 (purified) at 1/30 dilution (2ug) immunoprecipitating Lin28A in NCCIT whole cell lysate. NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab124765 & NCCIT whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124765 in NCCIT whole cell lysate

      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.

      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
      Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

      ab124765 (unpurified), at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    • Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)
      Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226089？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226089 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab226089.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com