Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Lin28A抗体[EPR4640] (ab124765)

Submit a review Q&A (4)References (11)
Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)
  • Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)
  • Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] (ab124765)
  • Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (ab124765)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)
  • Anti-Lin28A antibody [EPR4640] (ab124765)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4640] to Lin28A
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

概述

  • 产品名称

    Anti-Lin28A抗体[EPR4640]
    参阅全部 Lin28A 一抗

  • 描述

    兔单克隆抗体[EPR4640] to Lin28A

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: NCCIT and JAR whole cell lysates. IP: NCCIT whole cell lysates. Flow Cyt (intra): NCCIT cells. ICC/IF: JAR cells and NCCIT cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4640

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124765于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/500.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IP
1/10 - 1/100.
ICC/IF
1/100 - 1/500.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.

  • 组织特异性

    Expressed in embryonic stem cells (ES cells), placenta and testis.

  • 序列相似性

    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.

  • 发展阶段

    Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.

  • 结构域

    The CSD domain is required for function in muscle differentiation.

  • 细胞定位

    Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CSDD1 antibody
      • FLJ12457 antibody
      • LIN 28 antibody
      • Lin 28 homolog A (C. elegans) antibody
      • Lin-28A antibody
      • LIN28 antibody
      • LIN28A antibody
      • LN28A_HUMAN antibody
      • Protein lin-28 homolog A antibody
      • ZCCHC1 antibody
      • zinc finger CCHC domain containing 1 antibody
      • Zinc finger CCHC domain-containing protein 1 antibody
      see all

    图片

    • Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)
      Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)
      All lanes : Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution (Purified)

      Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates
      Lane 2 : JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 23 kDa
      Observed band size: 26 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)
      Western blot - Anti-Lin28A antibody [EPR4640] (ab124765)
      All lanes : Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution (unpurified)

      Lane 1 : NCCIT cell lysate
      Lane 2 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 23 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)
      Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)

      Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/500 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] (ab124765)
      Flow Cytometry (Intracellular) - Anti-Lin28A antibody [EPR4640] (ab124765)

      Intracellular Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/60 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (ab124765)
      Immunoprecipitation - Anti-Lin28A antibody [EPR4640] (ab124765)

      ab124765 (purified) at 1/30 dilution (2ug) immunoprecipitating Lin28A in NCCIT whole cell lysate. NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab124765 & NCCIT whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124765 in NCCIT whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)
      Immunocytochemistry/ Immunofluorescence - Anti-Lin28A antibody [EPR4640] (ab124765)

      ab124765 (unpurified), at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.

    • Anti-Lin28A antibody [EPR4640] (ab124765)
      Anti-Lin28A antibody [EPR4640] (ab124765)

    文献 (11)

    发表研究结果有使用 ab124765？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124765 被引用在 11 文献中.

    • Lee DW  et al. Antisense Oligonucleotides against Let-7 Enhance the Therapeutic Potential of Mesenchymal Stromal Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37239986
    • Dvir S  et al. Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells. Cell Rep 35:109198 (2021). PubMed: 34077720
    • Urresti J  et al. Cortical organoids model early brain development disrupted by 16p11.2 copy number variants in autism. Mol Psychiatry 26:7560-7580 (2021). PubMed: 34433918
    • Zhong Y  et al. Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression. Int J Biol Sci 16:1941-1953 (2020). PubMed: 32398961
    • Lin SL  et al. Overexpression of Lin28B Promoted the Proliferation of Adenomyotic Smooth Muscle Cells of the Junctional Zone via Regulating Let-7a. Reprod Sci 27:1156-1163 (2020). PubMed: 32046465
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Question

    Hello,

    Thank you for your reply.

    However, it is the DyLight 488 conjugated anti-Lin28A antibody [EPR4640] I am looking for.

    The Epitomics reference number is #4584-1.

    Do you now also have an abcam catalogue number for this item?

    Read More
    Answer

    Thank you for your email.

    I am sorry for the confusion cased but this is difference is simply from an error on our part in setting up the new datasheet. The product should be stated as being against LIN28A. This will be updated as soon as possible.

    The antibody ab139910 is directed against LIN28A (DyLight 488 conjugated) and is the same as the Epitomics product under the catalogue number 4584-1. They are both of the clone number EPR4640, the same as the unconjugated ab124765.

    I hope this has clarified things. If I can be of any further assistance, please do let me know.

    Read More

    Question

    Hello Karin,

    I have spoken to your colleague xxxxx about this item already – it will take a couple of weeks.

    However, please quote item number for your conjugation kit for DyLight 488 as we discussed.

    Med vänliga hälsningar/Kind regards

    Read More
    Answer

    I am sorry for the delay in getting back to you.

    I can now share further information with you in relation to the anti-Lin28A antibody [EPR4640] ab124765. This antibody is currently supplied at a concentration of 0.53 mg/mL. This is just within the recommended concentration to be used with the EasyLink conjugation kits.

    Alternatively, we now have the DyLight 488 conjugated version available from our catalogue under the catalogue number ab139910. If ordered today this should be with you in under 2 weeks.

    I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

    Read More

    Question

    Hello,

    I have spoken to your colleague xxxx about this item already – it will take a couple of weeks.

    However, please quote item number for your conjugation kit for DyLight 488 as we discussed.

    Read More
    Answer

    Thank you for your reply.

    The conjugation kits we have available can be accessed from the following link. I was incorrect to think we had DyLight 488 available but we have FITC which may be a suitable alternative?

    https://www.abcam.com/index.html?pageconfig=resource&rid=13148

    As the anti-Lin28A antibody [EPR4640] ab124765 is provided as a tissue culture supernatant we would recommend using the purification kit ab109207 to concentrate and purify the IgG content (the EasyLink kits are best used with antibody at a concentration of 0.5-5 mg/mL).

    I will enquire with Epitomics as to what the concentration of the antibody ab124765 is and will let you know as soon as I have this information.

    Read More

    Question

    Epitomics product 4584-1

    Read More
    Answer

    Thank you for contacting us.

    I have good news as well as bad news. Good news is that this product will be available on Abcam website and bad news - it may take 1-2 weeks, 2 weeks will be maximum.

    We have un-conjugated version ab124765, which is a Epitomics antibody if you are thinking about alternative.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com