Anti-LIMK2 (phospho T505)抗体(ab38499)
Key features and details
- Rabbit polyclonal to LIMK2 (phospho T505)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LIMK2 (phospho T505)抗体
参阅全部 LIMK2 一抗 -
描述
兔多克隆抗体to LIMK2 (phospho T505) -
宿主
Rabbit -
特异性
This antibody is specific for LIMK2 only when phosphorylated at threonine 505, but may also cross react with LIMK1 phosphorylated at tyrosine 505 based on homology. -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human LIMK-2 (phospho T505).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38499于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa). |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. -
组织特异性
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on serine and/or threonine residues by ROCK1. -
细胞定位
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3985 Human
- Omim: 601988 Human
- SwissProt: P53671 Human
- Unigene: 474596 Human
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别名
- LIM domain kinase 2 antibody
- LIM kinase 2 antibody
- LIMK 2 antibody
see all
图片
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Western blot - Anti-LIMK2 (phospho T505) antibody (ab38499)All lanes : Anti-LIMK2 (phospho T505) antibody (ab38499) at 1/500 dilution
Lane 1 : MCF7 cell extract (5-30ug of total protein) pre-incubated with phosphopeptide.
Lane 2 : MCF7 cell extract (5-30ug of total protein).
Secondary
All lanes : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 (phospho T505) antibody (ab38499)ab38499 at a 1:50 dilution staining LIMK2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 (phospho T505) antibody (ab38499)ICC/IF image of ab38499 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab38499 被引用在 16 文献中.
- Fang N & Li P O-linked N-acetylglucosaminyltransferase OGT inhibits diabetic nephropathy by stabilizing histone methyltransferases EZH2 via the HES1/PTEN axis. Life Sci 274:119226 (2021). PubMed: 33609540
- Gu Y et al. Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203. Aging (Albany NY) 12:2333-2346 (2020). PubMed: 32019904
- Xiao Y et al. Circular RNA Involved in the Protective Effect of Malva sylvestris L. on Myocardial Ischemic/Re-Perfused Injury. Front Pharmacol 11:520486 (2020). PubMed: 33101012
- Sousa-Squiavinato ACM et al. Cofilin-1 signaling mediates epithelial-mesenchymal transition by promoting actin cytoskeleton reorganization and cell-cell adhesion regulation in colorectal cancer cells. Biochim Biophys Acta Mol Cell Res 1866:418-429 (2019). PubMed: 30296500
- Zhang Z et al. MicroRNA-493-5p inhibits proliferation and metastasis of osteosarcoma cells by targeting Kruppel-like factor 5. J Cell Physiol 234:13525-13533 (2019). PubMed: 30773645