重组Anti-LIMK2抗体[EP969Y] - C-terminal (ab45165)
![Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)](https://www.abcam.cn/ps/products/45/ab45165/Images/ab45165-289257-anti-limk2-antibody-ep969y-conformation-specific-western-blot.jpg "Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP969Y] to LIMK2 - C-terminal
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LIMK2抗体[EP969Y] - C-terminal
参阅全部 LIMK2 一抗 -
描述
兔单克隆抗体[EP969Y] to LIMK2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HAP1 cell lysate. Jurkat cell lysate. Ramos membrane lysate. IHC-P: Human endometrium carcinoma tissue. ICC/IF: Ramos cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP969Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab45165于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| WB | (1) |
1/2000. Predicted molecular weight: 72 kDa.
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| 说明 |
|---|
|
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
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WB
1/2000. Predicted molecular weight: 72 kDa. |
靶标
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功能
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. -
组织特异性
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on serine and/or threonine residues by ROCK1. -
细胞定位
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3985 Human
- Entrez Gene: 16886 Mouse
- Entrez Gene: 29524 Rat
- Omim: 601988 Human
- SwissProt: P53671 Human
- SwissProt: O54785 Mouse
- SwissProt: P53670 Rat
- Unigene: 474596 Human
see all -
别名
- LIM domain kinase 2 antibody
- LIM kinase 2 antibody
- LIMK 2 antibody
see all
图片
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Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LIMK2 (LIMK2) knockout HAP1 whole cell lysate (40 µg)
Lane 3: Jurkat whole cell lysate (40 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab45165 was shown to specifically recognize LIMK2 (LIMK2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIMK2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)](https://www.abcam.cn/ps/products/45/ab45165/Images/ab45165-459765-anti-limk2-antibody-ep969y-c-terminal-immunohistochemistry-endometrium-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium cancer tissue labeling LIMK2 with ab45165 at 1/250 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)](https://www.abcam.cn/ps/products/45/ab45165/Images/ab45165-339543-ab45165.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)Immunocytochemistry/ Immunofluorescence analysis of Ramos cells labeling LIMK2 with ab45165 at 1/250. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI (blue) was used as a nuclear counterstain.
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![Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)](https://www.abcam.cn/ps/products/45/ab45165/Images/ab45165-57322-anti-limk2-antibody-ep969y-conformation-specific-western-blot.jpg)
Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165) at 1/2000 dilution + Ramos (Human Burkitt's lymphoma cell line) membrane lysate
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)](https://www.abcam.cn/ps/products/45/ab45165/Images/ab45165-6-benefits-of-recombinant-antibodies.png)
Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab45165 被引用在 8 文献中.
- Swiatlowska P et al. Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Sci Adv 8:eabm3471 (2022). PubMed: 35427166
- Yang W et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
- Duan X et al. The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis. J Cell Physiol 233:6088-6097 (2018). PubMed: 29319181
- Hyun HW et al. Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death. Front Cell Neurosci 10:99 (2016). PubMed: 27147971
- Ko AR et al. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 8:58 (2015). IHC-FoFr ; Rat . PubMed: 26438559
- Ray S et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). PubMed: 24966172
- Gamell C et al. LIMK2 mediates resistance to chemotherapeutic drugs in neuroblastoma cells through regulation of drug-induced cell cycle arrest. PLoS One 8:e72850 (2013). WB . PubMed: 23991158
- Rice DS et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS One 7:e47168 (2012). IHC-P ; Mouse . PubMed: 23071748