Anti-LIMD1抗体(ab225714)
Key features and details
- Rabbit polyclonal to LIMD1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LIMD1抗体
参阅全部 LIMD1 一抗 -
描述
兔多克隆抗体to LIMD1 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Guinea pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide within Human LIMD1 aa 50-100. The exact sequence is proprietary. (NP_055055.1)
Database link: Q9UGP4 -
阳性对照
- WB: HeLa, Jurkat and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225714于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
|
| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 72 kDa. |
靶标
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功能
Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains. -
细胞定位
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli. - Information by UniProt
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数据库链接
- Entrez Gene: 8994 Human
- Omim: 604543 Human
- SwissProt: Q9UGP4 Human
- Unigene: 193370 Human
- Unigene: 621057 Human
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别名
- AW822033 antibody
- D9Ertd192e antibody
- LIM domain-containing protein 1 antibody
see all
图片
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Western blot - Anti-LIMD1 antibody (ab225714)All lanes : Anti-LIMD1 antibody (ab225714) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-LIMD1 antibody (ab225714)LIMD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225714 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225714 at 0.4 µg/ml.
Lane 1: ab225714 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225714 尚未被引用在任何文献中。