重组Anti-LIM Kinase 1抗体[EPR912] (ab108507)
![Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)](https://www.abcam.cn/ps/products/108/ab108507/Images/ab108507-551222-anti-lim-kinase-1antibody-epr912-western-blot-wild-type-hap1-cells-human.jpg "Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR912] to LIM Kinase 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LIM Kinase 1抗体[EPR912]
参阅全部 LIM Kinase 1 一抗 -
描述
兔单克隆抗体[EPR912] to LIM Kinase 1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- U-87 MG, SH SY5Y and Ramos cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR912 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
靶标
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功能
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. -
组织特异性
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. -
疾病相关
Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
细胞定位
Cytoplasm. Cell projection > growth cone. - Information by UniProt
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数据库链接
- Entrez Gene: 3984 Human
- Omim: 601329 Human
- SwissProt: P53667 Human
- Unigene: 647035 Human
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别名
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
图片
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Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/500 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : LIMK1 knockout HAP1 cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 73 kDaLanes 1 - 2: Merged signal (red and green). Green - ab108507 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
In Western blot: ab108507 was shown to specifically react with LIM Kinase 1 when LIM Kinase 1 knockout samples were used. Wild-type and LIM Kinase 1 knockout samples were subjected to SDS-PAGE. ab108507 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)](https://www.abcam.cn/ps/products/108/ab108507/Images/ab108507-121205-anti-lim-kinase-1-antibody-epr912-western-blot.jpg)
Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507)All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa -
![Anti-LIM Kinase 1 antibody [EPR912] (ab108507)](https://www.abcam.cn/ps/products/108/ab108507/Images/ab108507-3-benefits-of-recombinant-antibodies.png)
Anti-LIM Kinase 1 antibody [EPR912] (ab108507)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108507 被引用在 5 文献中.
- Feng Y et al. Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis. Cell Mol Life Sci 79:498 (2022). PubMed: 36008729
- Pang D et al. Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-?B pathway. Biol Open 9:N/A (2020). PubMed: 31988091
- Zhu Q et al. IRX5 promotes colorectal cancer metastasis by negatively regulating the core components of the RHOA pathway. Mol Carcinog 58:2065-2076 (2019). PubMed: 31432570
- Duan X et al. The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis. J Cell Physiol 233:6088-6097 (2018). PubMed: 29319181
- Gu H et al. Genistein up-regulates miR-20a to disrupt spermatogenesis via targeting Limk1. Oncotarget 8:58728-58737 (2017). WB ; Mouse . PubMed: 28938591