AB108507

重组Anti-LIM Kinase 1抗体[EPR912]

Anti-LIM Kinase 1 antibody [EPR912]

RabMAb
Recombinant
KO Validated
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(6 Publications)

Rabbit Recombinant Monoclonal LIM Kinase 1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

查看别名

LIMK, LIMK1, LIM domain kinase 1, LIMK-1

1 Images

Unknown

Western blot - Anti-LIM Kinase 1 antibody [EPR912] (AB108507)

All lanes:

Western blot - Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution

Lane 1:

U-87 MG cell lysate at 10 µg

Lane 2:

SH SY5Y cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Predicted band size: 73 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-LIM Kinase 1 antibody [EPR912] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR912

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LIM Kinase 1 also known as LIMK1 is a serine/threonine protein kinase that plays a critical role in regulating actin cytoskeleton dynamics. Weighing approximately 72 kDa LIMK1 is expressed in various tissues with significant levels observed in the brain and spinal cord. Some studies highlight its elevated presence in neurons. The LIMK1 gene encodes the protein and its activity is often regulated through phosphorylation processes. Researchers often refer to related proteins like LIMK1/proteina in discussions about similar functions.

Biological function summary

This kinase modulates cell shape migration and differentiation by influencing actin filament networks. LIMK1 associates with regulatory complexes and interacts with several proteins to facilitate cytoskeletal reorganization. It plays an essential part in neural development and synaptic plasticity affecting learning and memory. By phosphorylating key actin-binding proteins like cofilin LIMK1 alters actin dynamics essential for various cellular functions.

Pathways

LIM Kinase 1 influences the RhoA/ROCK and Rac1/Pak pathways both critical for cytoskeletal rearrangements. Within these pathways LIMK1 interacts closely with proteins such as cofilin and is influenced by upstream activators like Rho GTPases. These pathways link it to a broader network of signaling that controls cell movement and stability important for cellular responses in various environmental contexts.

LIMK1 is linked to neurological conditions like Williams-Beuren syndrome and some forms of intellectual disability. Changes in LIMK1 expression or activity can disrupt normal brain function leading to cognitive deficits. Its connection to cofilin and other cytoskeleton-associated proteins plays a role in the pathological development of these conditions underlining its importance in maintaining normal neuronal architecture and connectivity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed : 10436159, PubMed : 11832213, PubMed : 12807904, PubMed : 15660133, PubMed : 16230460, PubMed : 18028908, PubMed : 22328514, PubMed : 23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PubMed : 10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton (PubMed : 11832213, PubMed : 15660133, PubMed : 16230460, PubMed : 23633677). In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation (PubMed : 11832213, PubMed : 15660133, PubMed : 16230460, PubMed : 23633677). Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly (PubMed : 18028908). Stimulates axonal outgrowth and may be involved in brain development (PubMed : 18028908).. Isoform 3. Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

See full target information LIMK1

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 54: PubMed39301627

2024

Molecular mechanisms and clinicopathological characteristics of inhibin βA in thyroid cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wanjun Zhao,Weiyu Wang,Yifan Zhu,Zhenghua Lv,Wei Xu

Cellular and molecular life sciences : CMLS 79:498 PubMed36008729

2022

Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Feng,Dake Chen,Tiansu Wang,Jiawei Zhou,Wenning Xu,Hao Xiong,Rong Bai,Shang Wu,Jialian Li,Fenge Li

Biology open 9: PubMed31988091

2020

Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dejiang Pang,Chengcheng Yang,Chao Li,Yuanfeng Zou,Bin Feng,Lixia Li,Wentao Liu,Qihui Luo,Zhengli Chen,Chao Huang

Molecular carcinogenesis 58:2065-2076 PubMed31432570

2019

IRX5 promotes colorectal cancer metastasis by negatively regulating the core components of the RHOA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiangqiang Zhu,Yiqi Wu,Mengli Yang,Zhen Wang,Hailing Zhang,Xinying Jiang,Meng Chen,Tianyu Jin,Ting Wang

Journal of cellular physiology 233:6088-6097 PubMed29319181

2018

The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Duan,Yu Zhang,Kun-Lin Chen,Hao-Lin Zhang,Lan-Lan Wu,Hong-Lin Liu,Zhen-Bo Wang,Shao-Chen Sun

Oncotarget 8:58728-58737 PubMed28938591

2017

Genistein up-regulates miR-20a to disrupt spermatogenesis via targeting Limk1.

Applications

Species

Mouse

Hao Gu,Wei Wu,Beilei Yuan,Qiuqin Tang,Dan Guo,Yiqiu Chen,Yankai Xia,Lingqing Hu,Daozhen Chen,Jiahao Sha,Xinru Wang

View all publications

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重组Anti-LIM Kinase 1抗体[EPR912]