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AB313613

重组Anti-LILRB5抗体[EPR23896-84] - BSA and Azide free

Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free

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Rabbit Recombinant Monoclonal LILRB5 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Transfected cell lysate - Human, Transfected cell line - Human samples.

查看别名

CD85c, LIR8, LILRB5, Leukocyte immunoglobulin-like receptor subfamily B member 5, CD85 antigen-like family member C, Leukocyte immunoglobulin-like receptor 8, LIR-8

7 Images
Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) cells labelling LILRB5 with ab313612 at 1/50 dilution (1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized human PBMC (human peripheral blood mononuclear cell) cells labelling LILRB5 with ab313612 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human LILRB5 expression vector containing a myc tag (Right) cells labelling LILRB5 with ab313612 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-Myc conjugated to 647.

Immunocytochemistry/ Immunofluorescence - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling LILRB5 with ab313612 at 1/2000 (0.255 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in 293T cells transfected with a human LILRB5 expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling LILRB5 with ab313612 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Western blot - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • WB

Supplier Data

Western blot - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : 293T. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 114 seconds

All lanes:

Western blot - Anti-LILRB5 antibody [EPR23896-84] (<a href='/products/primary-antibodies/lilrb5-antibody-epr23896-84-ab313612'>ab313612</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 60 µg

Lane 2:

No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 114s

Western blot - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)
  • WB

Supplier Data

Western blot - Anti-LILRB5 antibody [EPR23896-84] - BSA and Azide free (AB313613)

This data was developed using ab313612, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with human LILRB1, LILRB2, LILRB3, and LILRB4. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 103 seconds

All lanes:

Western blot - Anti-LILRB5 antibody [EPR23896-84] (<a href='/products/primary-antibodies/lilrb5-antibody-epr23896-84-ab313612'>ab313612</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag whole cell lysate at 60 µg

Lane 2:

293T cells transfected with a human LILRB3 expression vector containing a His-tag whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a human LILRB1 expression vector containing a His-tag whole cell lysate at 5 µg

Lane 4:

293T cells transfected with a human LILRB5 expression vector containing a His-tag whole cell lysate at 60 µg

Lane 5:

293T cells transfected with a human LILRB2 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a human LILRB4 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 103s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23896-84

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The LILRB5 protein also known as Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 5 has an approximate mass of 65 kDa. It belongs to the immunoglobulin superfamily and is primarily found on the surface of several immune cells including monocytes and macrophages. LILRB5 plays a significant role in down-modulating immune responses by interacting with its ligands.
Biological function summary

This protein functions as an inhibitory receptor that modulates immune cell activation and responses. It is not part of a larger protein complex but works individually to regulate anti-inflammatory signaling pathways. Its activity helps maintain immune homeostasis and prevents overactivation of immune responses which can lead to tissue damage.

Pathways

LILRB5 acts within the context of immune response regulation. It is involved in pathways related to immunosuppressive signaling notably the PD-1 receptor pathway. LILRB5 interacts with other immune-regulating proteins like LILRB1 and PD-L1 which coordinate to control immune checkpoint signaling suppressing excessive immune reactions.

LILRB5 has connections to autoimmune diseases and cancer. Its role in suppressing immune responses links it to autoimmune conditions where inappropriate targeting of self-tissues occurs suggesting that its dysregulation may lead to such disorders. Furthermore in cancer tumor cells might exploit LILRB5-mediated pathways to evade immune surveillance. Related proteins like LILRB1 and PD-L1 further complicate these pathways influencing disease progression and therapeutic outcomes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

May act as receptor for class I MHC antigens.
See full target information LILRB5
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Abcam Product Promise

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