AB177946

重组Anti-LIG1抗体[EPR12464]

Name: LIG1抗体[EPR12464] (ab177946)| Abcam中文官网
Brand: Abcam Limited
SKU: AB177946
Price: 2854 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-LIG1 antibody [EPR12464]

RabMAb
Recombinant
了解详情

(2 Reviews)

(9 Publications)

Rabbit Recombinant Monoclonal LIG1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 9 publications.

查看别名

DNA ligase 1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1, LIG1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody [EPR12464] (AB177946)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LIG1 antibody [EPR12464] (AB177946)

Immunofluorescent analysis of HeLa cells labeling LIG1 with ab177946 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (AB177946)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (AB177946)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling LIG1 with ab177946 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIG1 antibody [EPR12464] (AB177946)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling LIG1 with ab177946 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-LIG1 antibody [EPR12464] (AB177946)

All lanes:

Western blot - Anti-LIG1 antibody [EPR12464] (ab177946) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Molt 4 cell lysate at 10 µg

Predicted band size: 102 kDa

false

不同偶联物与剂型 (8)

Carrier free

Anti-LIG1 antibody [EPR12464] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-LIG1 antibody [EPR12464]
660 APC

APC Anti-LIG1 antibody [EPR12464]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-LIG1 antibody [EPR12464]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-LIG1 antibody [EPR12464]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-LIG1 antibody [EPR12464]
578 PE

PE Anti-LIG1 antibody [EPR12464]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-LIG1 antibody [EPR12464]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12464

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 'LIG1' protein also known as DNA ligase I plays an important role in DNA replication and repair. Mechanically LIG1 is responsible for catalyzing the formation of phosphodiester bonds between adjacent DNA strands an essential step for sealing Okazaki fragments during lagging strand synthesis. This protein features a molecular weight of approximately 102 kDa. LIG1 is predominantly expressed in the nucleus of proliferating cells with presence noted in various tissues including those undergoing rapid cell division.

Biological function summary

DNA ligase I which is sometimes part of larger DNA replication complexes ensures the faithful duplication and repair of the genome. This protein assists with repairing single and double-strand breaks thereby maintaining genomic stability. In the context of DNA replication LIG1 is necessary for completing the joining of discontinuous segments preventing loss of genetic information. Mutations or deficiencies can compromise this repairing process potentially leading to genome instability.

Pathways

LIG1 participates in the DNA replication pathway and the base excision repair pathway. Within these pathways LIG1 interacts with other proteins such as proliferating cell nuclear antigen (PCNA) to facilitate its recruitment to DNA and enhance its ligation efficiency. Additionally LIG1 associates with replication factor C (RFC) which acts as a clamp loader and flap endonuclease 1 (FEN1) during DNA replication and repair ensuring proper genome maintenance.

Defective LIG1 function relates to genomic instability which can contribute to conditions such as immunodeficiency and increased cancer susceptibility. For example mutations in the LIG1 gene have been associated with Ligase I deficiency which presents with hypersensitivity to DNA-damaging agents and immunodeficiency. LIG1's activity is also connected to Bloom syndrome where defective DNA repair leads to chromosomal instability involving interactions with proteins such as the Bloom syndrome protein (BLM).

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

DNA ligase that seals nicks in double-stranded during DNA repair (PubMed : 30395541). Also involved in DNA replication and DNA recombination.

See full target information LIG1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:5237 PubMed38898005

2024

Therapy-induced secretion of spliceosomal components mediates pro-survival crosstalk between ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria O Shender,Ksenia S Anufrieva,Polina V Shnaider,Georgij P Arapidi,Marat S Pavlyukov,Olga M Ivanova,Irina K Malyants,Grigory A Stepanov,Evgenii Zhuravlev,Rustam H Ziganshin,Ivan O Butenko,Olga N Bukato,Ksenia M Klimina,Vladimir A Veselovsky,Tatiana V Grigorieva,Sergey Y Malanin,Olga I Aleshikova,Andrey V Slonov,Nataliya A Babaeva,Lev A Ashrafyan,Elena Khomyakova,Evgeniy G Evtushenko,Maria M Lukina,Zixiang Wang,Artemiy S Silantiev,Anna A Nushtaeva,Daria D Kharlampieva,Vassili N Lazarev,Arseniy I Lashkin,Lorine K Arzumanyan,Irina Yu Petrushanko,Alexander A Makarov,Olga S Lebedeva,Alexandra N Bogomazova,Maria A Lagarkova,Vadim M Govorun

NPJ precision oncology 6:51 PubMed35853939

2022

Targeting Mre11 overcomes platinum resistance and induces synthetic lethality in XRCC1 deficient epithelial ovarian cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Adel Alblihy,Reem Ali,Mashael Algethami,Ahmed Shoqafi,Michael S Toss,Juliette Brownlie,Natalie J Tatum,Ian Hickson,Paloma Ordonez Moran,Anna Grabowska,Jennie N Jeyapalan,Nigel P Mongan,Emad A Rakha,Srinivasan Madhusudan

G3 (Bethesda, Md.) 12: PubMed35666183

2022

Integrative analysis and prediction of human R-loop binding proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Arun Kumar,Louis-Alexandre Fournier,Peter C Stirling

Nature 606:930-936 PubMed35477155

2022

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shangming Tang,Ema Stokasimov,Yuxiang Cui,David Pellman

Cell death and differentiation 29:1639-1653 PubMed35194187

2022

Human cytomegalovirus hijacks host stress response fueling replication stress and genome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Maria Merchut-Maya,Jiri Bartek,Jirina Bartkova,Panagiotis Galanos,Mattia Russel Pantalone,MyungHee Lee,Huanhuan L Cui,Patrick J Shilling,Christian Beltoft Brøchner,Helle Broholm,Apolinar Maya-Mendoza,Cecilia Söderberg-Naucler,Jiri Bartek

Placenta 106:7-14 PubMed33601221

2021

Downregulation of DNA ligases in trophoblasts contributes to recurrent pregnancy loss through inducing DNA damages.

Applications

Unspecified application

Species

Unspecified reactive species

Peng-Fen Li,Yun-Gai Xiang,Dan Zhang,Na Lu,Qian Dou,Li Tan

The Journal of clinical investigation 128:5489-5504 PubMed30395541

2018

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Maffucci,Jose Chavez,Thomas J Jurkiw,Patrick J O'Brien,Jordan K Abbott,Paul R Reynolds,Austen Worth,Luigi D Notarangelo,Kerstin Felgentreff,Patricia Cortes,Bertrand Boisson,Lin Radigan,Aurélie Cobat,Chitra Dinakar,Mohammad Ehlayel,Tawfeg Ben-Omran,Erwin W Gelfand,Jean-Laurent Casanova,Charlotte Cunningham-Rundles

Nature communications 9:335 PubMed29362359

2018

Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation.

Applications

Species

Unspecified reactive species

Marcel Naumann,Arun Pal,Anand Goswami,Xenia Lojewski,Julia Japtok,Anne Vehlow,Maximilian Naujock,René Günther,Mengmeng Jin,Nancy Stanslowsky,Peter Reinhardt,Jared Sterneckert,Marie Frickenhaus,Francisco Pan-Montojo,Erik Storkebaum,Ina Poser,Axel Freischmidt,Jochen H Weishaupt,Karlheinz Holzmann,Dirk Troost,Albert C Ludolph,Tobias M Boeckers,Stefan Liebau,Susanne Petri,Nils Cordes,Anthony A Hyman,Florian Wegner,Stephan W Grill,Joachim Weis,Alexander Storch,Andreas Hermann

Nature communications 8:1470 PubMed29133916

2017

BRCA2 antagonizes classical and alternative nonhomologous end-joining to prevent gross genomic instability.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Han,Chunyan Ruan,Michael S Y Huen,Jiadong Wang,Anyong Xie,Chun Fu,Ting Liu,Jun Huang

View all publications

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重组Anti-LIG1抗体[EPR12464]