重组Anti-LIFR抗体[EPR24651-141] - BSA and Azide free (Capture) (ab317468)
![Sandwich ELISA - Anti-LIFR antibody [EPR24651-141] - BSA and Azide free (Capture) (ab317468)](https://www.abcam.cn/ps/products/317/ab317468/Images/ab317468-2-anti-lifr-antibody-pair-sandwich-elisa-epr24651-141-epr24651-35.jpg "Sandwich ELISA - Anti-LIFR antibody [EPR24651-141] - BSA and Azide free (Capture) (ab317468)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24651-141] to LIFR - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-LIFR抗体[EPR24651-141] - BSA and Azide free (Capture)
参阅全部 LIFR 一抗 -
描述
兔单克隆抗体[EPR24651-141] to LIFR - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab317468 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LIFR. The recommended pair for sandwich ELISA is:
Capture: ab317468, Human LIFR Capture Antibody (unconjugated)
Detector: ab317469, Human LIFR Detector Antibody (unconjugated)
The reference range value is 78.13-5000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24651-141 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317468于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24651-35] to LIFR - BSA and Azide free (Detector) (ab317469).
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24651-35] to LIFR - BSA and Azide free (Detector) (ab317469). |
靶标
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功能
Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells. -
疾病相关
Defects in LIFR are the cause of Stueve-Wiedemann syndrome (SWS) [MIM:601559]; also knowns as Schwartz-Jampel syndrome type 2 (SJS2). SWS is a severe autosomal recessive condition and belongs to the group of the bent-bone dysplasias. SWS is characterized by bowing of the lower limbs, with internal cortical thickening, wide metaphyses with abnormal trabecular pattern, and camptodactyly. Additional features include feeding and swallowing difficulties, as well as respiratory distress and hyperthermic episodes, which cause death in the first months of life. The rare survivors develop progressive scoliosis, spontaneous fractures, bowing of the lower limbs, with prominent joints and dysautonomia symptoms, including temperature instability, absent corneal and patellar reflexes, and smooth tongue.
Note=A chromosomal aberration involving LIFR is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(5;8)(p13;q12) with PLAG1. -
序列相似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 6 fibronectin type-III domains. -
结构域
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3977 Human
- Omim: 151443 Human
- SwissProt: P42702 Human
- Unigene: 133421 Human
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别名
- CD118 antibody
- CD118 antigen antibody
- FLJ98106 antibody
see all
图片
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Sandwich ELISA - Anti-LIFR antibody [EPR24651-141] - BSA and Azide free (Capture) (ab317468)
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![Anti-LIFR antibody [EPR24651-141] - BSA and Azide free (Capture) (ab317468)](https://www.abcam.cn/ps/products/317/ab317468/Images/ab317468-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-LIFR antibody [EPR24651-141] - BSA and Azide free (Capture) (ab317468)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab317468 尚未被引用在任何文献中。
