Anti-LIF抗体(ab113262)
Key features and details
- Rabbit polyclonal to LIF
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-LIF抗体
参阅全部 LIF 一抗 -
描述
兔多克隆抗体to LIF -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
A 16 amino acid synthetic peptide corresponding to internal sequence amino acids of Human LIF (NP_002300).
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阳性对照
- WB: NIH/3T3 and EL4 cell lysates. Mouse colon and rat lung tissue lysates; ICC/IF: NIH/3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab113262 is purified by affinity chromatography via a peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa). |
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ICC/IF
Use a concentration of 20 µg/ml. |
靶标
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功能
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
序列相似性
Belongs to the LIF/OSM family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 16878 Mouse
- Entrez Gene: 60584 Rat
- SwissProt: P09056 Mouse
- SwissProt: P17777 Rat
- Unigene: 4964 Mouse
- Unigene: 44379 Rat
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别名
- CDF antibody
- Cholinergic Differentiation Factor antibody
- D factor antibody
see all
图片
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Western blot - Anti-LIF antibody (ab113262)All lanes : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : EL4 (mouse lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-LIF antibody (ab113262)Immunofluorescence analysis of 3T3 cells, staining LIF with ab113262 at 20 µg/ml. -
Western blot - Anti-LIF antibody (ab113262)Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa -
Western blot - Anti-LIF antibody (ab113262)Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : Rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab113262 被引用在 19 文献中.
- Chen X et al. Interferon regulatory factor 1 (IRF1) inhibits lung endothelial regeneration following inflammation-induced acute lung injury. Clin Sci (Lond) 137:367-383 (2023). PubMed: 36857175
- Miyazaki Y et al. Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts. Cancers (Basel) 14:N/A (2022). PubMed: 35326527
- Chen Q et al. Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration. Cell Death Dis 12:417 (2021). PubMed: 33888681
- Wang G et al. Effects of bone marrow mesenchymal stem cells on repair and receptivity of damaged endometrium in rats. J Obstet Gynaecol Res 47:3223-3231 (2021). PubMed: 34184363
- Xie K et al. Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia. Cancer Cell Int 21:627 (2021). PubMed: 34838029