Anti-LGR5抗体[OTI2A2] (ab273092)
![Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)](https://www.abcam.cn/ps/products/273/ab273092/Images/ab273092-390949-anti-lrg5-antibody-flow-cytometry-hek239-cells.JPG "Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)")
Key features and details
- Mouse monoclonal [OTI2A2] to LGR5
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-LGR5抗体[OTI2A2]
参阅全部 LGR5 一抗 -
描述
小鼠单克隆抗体[OTI2A2] to LGR5 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human LGR5 aa 250-550. Genbank: NP_003658)
Database link: O75473 -
阳性对照
- Flow Cyt: HEK-293T cells transfected with pCMV6-ENTRY LGR5 cDNA ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY LGR5. HT-29 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
OTI2A2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/100.
|
|
| ICC/IF |
1/100.
|
| 说明 |
|---|
|
Flow Cyt
1/100. |
|
ICC/IF
1/100. |
靶标
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功能
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling. -
组织特异性
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. -
序列相似性
Belongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8549 Human
- Omim: 606667 Human
- SwissProt: O75473 Human
- Unigene: 658889 Human
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别名
- FEX antibody
- G protein coupled receptor 49 antibody
- G protein coupled receptor 67 antibody
see all
图片
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Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)
Flow cytometric analysis of HEK-293T cells transfected with either LGR5 overexpressed plasmid (Red) or empty vector control plasmid (Blue). Cells were immunostained with ab273092 at 1/100 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)](https://www.abcam.cn/ps/products/273/ab273092/Images/ab273092-390950-anti-lrg5-antibody-immuocytochemistry-ht29-cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)Immunofluorescent staining of HT-29 cells labeling LGR5 using ab273092 at 1/100 dilution (green). Actin filaments were labeled with TRITC-phalloidin (red). The nuclear counter stain is DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)](https://www.abcam.cn/ps/products/273/ab273092/Images/ab273092-390953-anti-lrg5-antibody-immuocytochemistry-cos7cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LGR5, labeling LGR5 using ab273092 at 1/100 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab273092 被引用在 6 文献中.
- Sun Q et al. Antimicrobial agent chloroxylenol targets β‑catenin‑mediated Wnt signaling and exerts anticancer activity in colorectal cancer. Int J Oncol 63:N/A (2023). PubMed: 37681484
- Han L et al. Cell transcriptomic atlas of the non-human primate Macaca fascicularis. Nature 604:723-731 (2022). PubMed: 35418686
- Zheng H et al. Characterization of stem cell landscape and identification of stemness-relevant prognostic gene signature to aid immunotherapy in colorectal cancer. Stem Cell Res Ther 13:244 (2022). PubMed: 35681225
- Zhu T et al. D-galactose protects the intestine from ionizing radiation-induced injury by altering the gut microbiome. J Radiat Res 63:805-816 (2022). PubMed: 36253108
- Yu W et al. LGR5 enhances the osteoblastic differentiation of MC3T3-E1 cells through the Wnt/β-catenin pathway. Exp Ther Med 22:889 (2021). PubMed: 34194567
- Liang Y et al. Beta-1 syntrophin (SNTB1) regulates colorectal cancer progression and stemness via regulation of the Wnt/β-catenin signaling pathway. Ann Transl Med 9:1016 (2021). PubMed: 34277816