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AB273092

Anti-LGR5抗体[OTI2A2]

Anti-LGR5 antibody [OTI2A2]

5

(1 Review)

|

(11 Publications)

Anti-LGR5 antibody [OTI2A2] (ab273092) is a mouse monoclonal antibody detecting LGR5 in Flow Cytometry, ICC/IF. Suitable for Human,.

查看别名

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (AB273092)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (AB273092)

Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LGR5, labeling LGR5 using ab273092 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (AB273092)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (AB273092)

Immunofluorescent staining of HT-29 cells labeling LGR5 using ab273092 at 1/100 dilution (green). Actin filaments were labeled with TRITC-phalloidin (red). The nuclear counter stain is DAPI (blue).

Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (AB273092)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (AB273092)

Flow cytometric analysis of HEK-293T cells transfected with either LGR5 overexpressed plasmid (Red) or empty vector control plasmid (Blue). Cells were immunostained with ab273092 at 1/100 dilution.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI2A2

亚型

IgG1

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, Flow Cyt

applications

免疫原

Recombinant Fragment Protein within Human LGR5 aa 250-550. The exact immunogen used to generate this antibody is proprietary information.

O75473

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替代产品

Primary Antibodies

AB75850

Anti-LGR5 antibody [EPR3065Y]

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Western blot - Anti-LGR5 antibody [EPR3065Y] (AB75850)

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Primary Antibodies

AB219107

Anti-LGR5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody (AB219107)

  • 4
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搭配使用产品

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Anti-Ki67 antibody [SP6]

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Primary Antibodies

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Anti-Lysozyme antibody [EPR2994(2)]

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Primary Antibodies

AB282457

Anti-EpCAM antibody [RM1016]

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Immunocytochemistry - Anti-EpCAM antibody [RM1016] (AB282457)

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Primary Antibodies

AB185966

Anti-SOX9 antibody [EPR14335-78]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (AB185966)

  • 5
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Product Specifications
Anti-LGR5 antibody [OTI2A2] (ab273092) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF in human samples.
Anti-LGR5 antibody [OTI2A2] (ab273092) specifically detects LGR5 (UniProt ID: O75473; Molecular weight: 98kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-LGR5 antibody [OTI2A2] (ab273092) has high sensitivity and specificity.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
Purified from tissue culture supernatant.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
See full target information LGR5
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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:878 PubMed39632825

2024

Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

A Esposito,A Agostini,G Quero,G Piro,L Priori,A Caggiano,G Scaglione,A Battaglia,M A Calegari,L Salvatore,M Bensi,M G Maratta,A Ceccarelli,G Trovato,G Genovese,E Gurreri,S Ascrizzi,M Martini,C Fiorillo,A Fattorossi,F De Sanctis,S Ugel,V Corbo,S Alfieri,G Tortora,C Carbone

Molecules and cells 47:100074 PubMed38901530

2024

Targeting of CYP2E1 by miRNAs in alcohol-induced intestine injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hyejin Mun,Sungyul Lee,Suyoung Choi,Ji-Hoon Jeong,Seungbeom Ko,Yoo Lim Chun,Benjamin Deaton,Clay T Yeager,Audrey Boyette,Juliana Palmera,London Newman,Ping Zhou,Soona Shin,Dong-Chan Kim,Cari A Sagum,Mark T Bedford,Young-Kook Kim,Jaeyul Kwon,Junyang Jung,Jeong Ho Chang,Je-Hyun Yoon

Protein & cell 15:530-546 PubMed38486356

2024

Comprehensive transcriptional atlas of human adenomyosis deciphered by the integration of single-cell RNA-sequencing and spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Yiliang Xu,Xiaocui Xu,Jianzhang Wang,Zhiruo Qiu,Yayuan Yu,Xiaohong Jiang,Wanqi Shao,Dandan Bai,Mingzhu Wang,Shuyan Mei,Tao Cheng,Li Wu,Shaorong Gao,Xuan Che

Biomedicines 11: PubMed38137414

2023

Application of a Receptor-Binding-Domain-Based Simple Immunoassay for Assessing Humoral Immunity against Emerging SARS-CoV-2 Virus Variants.

Applications

Unspecified application

Species

Unspecified reactive species

Orsolya Mózner,Judit Moldvay,Kata Sára Szabó,Dorottya Vaskó,Júlia Domján,Dorottya Ács,Zoltán Ligeti,Csaba Fehér,Edit Hirsch,László Puskás,Cordula Stahl,Manfred Frey,Balázs Sarkadi

International journal of oncology 63: PubMed37681484

2023

Antimicrobial agent chloroxylenol targets β‑catenin‑mediated Wnt signaling and exerts anticancer activity in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Boxin Liu,Quanxue Lan,Zijie Su,Qiuxia Fu,Lian Wang,Yingying Deng,Chuanli Li,Vivian Weiwen Xue,Shanshan Liu,Xianxiong Chen,Guowu Yang,Desheng Lu

Journal of radiation research 63:805-816 PubMed36253108

2022

D-galactose protects the intestine from ionizing radiation-induced injury by altering the gut microbiome.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Zhu,Zhouxuan Wang,Junbo He,Xueying Zhang,Changchun Zhu,Shuqin Zhang,Yuan Li,Saijun Fan

Stem cell research & therapy 13:244 PubMed35681225

2022

Characterization of stem cell landscape and identification of stemness-relevant prognostic gene signature to aid immunotherapy in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zheng,Heshu Liu,Huayu Li,Weidong Dou,Jingui Wang,Junling Zhang,Tao Liu,Yingchao Wu,Yucun Liu,Xin Wang

Clinical science (London, England : 1979) 136:657-673 PubMed35475476

2022

ANGPTL1 attenuates cancer migration, invasion, and stemness through regulating FOXO3a-mediated SOX2 expression in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Chang,Kuo-Cheng Lan,Chen-Yuan Chiu,Meei-Ling Sheu,Shing-Hwa Liu

Nature 604:723-731 PubMed35418686

2022

Cell transcriptomic atlas of the non-human primate Macaca fascicularis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Han,Xiaoyu Wei,Chuanyu Liu,Giacomo Volpe,Zhenkun Zhuang,Xuanxuan Zou,Zhifeng Wang,Taotao Pan,Yue Yuan,Xiao Zhang,Peng Fan,Pengcheng Guo,Yiwei Lai,Ying Lei,Xingyuan Liu,Feng Yu,Shuncheng Shangguan,Guangyao Lai,Qiuting Deng,Ya Liu,Liang Wu,Quan Shi,Hao Yu,Yunting Huang,Mengnan Cheng,Jiangshan Xu,Yang Liu,Mingyue Wang,Chunqing Wang,Yuanhang Zhang,Duo Xie,Yunzhi Yang,Yeya Yu,Huiwen Zheng,Yanrong Wei,Fubaoqian Huang,Junjie Lei,Waidong Huang,Zhiyong Zhu,Haorong Lu,Bo Wang,Xiaofeng Wei,Fengzhen Chen,Tao Yang,Wensi Du,Jing Chen,Shibo Xu,Juan An,Carl Ward,Zongren Wang,Zhong Pei,Chi-Wai Wong,Xiaolei Liu,Huafeng Zhang,Mingyuan Liu,Baoming Qin,Axel Schambach,Joan Isern,Liqiang Feng,Yan Liu,Xiangyu Guo,Zhen Liu,Qiang Sun,Patrick H Maxwell,Nick Barker,Pura Muñoz-Cánoves,Ying Gu,Jan Mulder,Mathias Uhlen,Tao Tan,Shiping Liu,Huanming Yang,Jian Wang,Yong Hou,Xun Xu,Miguel A Esteban,Longqi Liu

Annals of translational medicine 9:1016 PubMed34277816

2021

Beta-1 syntrophin (SNTB1) regulates colorectal cancer progression and stemness via regulation of the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfang Liang,Bin Wang,Shasha Chen,Ziyu Ye,Xingxing Chai,Ronggang Li,Xiaoping Li,Gang Kong,Yanyun Li,Xueying Zhang,Zhengping Che,Qi Xie,Jiachun Lian,Bihua Lin,Xin Zhang,Xueqin Huang,Weijuan Huang,Xianxiu Qiu,Jincheng Zeng
View all publications
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