AB240101

重组Anti-Lgi1/EPT抗体[EPR9084] - BSA and Azide free

Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Lgi1/EPT antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

查看别名

EPT, UNQ775/PRO1569, LGI1, Leucine-rich glioma-inactivated protein 1, Epitempin-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Lgi1/EPT with ab137045 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137045).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

This data was developed using ab137045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining in mouse cerebrum.
The section was incubated with ab137045 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

This data was developed using ab137045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat kidney labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Negative control : no staining on rat kidney.
The section was incubated with ab137045 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

This data was developed using ab137045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Negative control : no staining on mouse kidney.
The section was incubated with ab137045 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

This data was developed using ab137045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum labeling Lgi1/EPT with ab137045 at 1/300 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining in rat cerebrum.
The section was incubated with ab137045 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Lab

Western blot - Anti-Lgi1/EPT antibody [EPR9084] - BSA and Azide free (AB240101)

This data was developed using ab137045, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart, kidney, spleen.

The identity of the bands lower than 25 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Lgi1/EPT antibody [EPR9084] (<a href='/products/primary-antibodies/lgi1-ept-antibody-epr9084-ab137045'>ab137045</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 64 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Unconjugated

Anti-Lgi1/EPT antibody [EPR9084]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9084

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab240101 is the carrier-free version of ab137045.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Lgi1 also known as leucine-rich glioma inactivated 1 is a synaptic protein with a mass of approximately 60 kDa. This protein is notably expressed in the brain where it plays a mechanical role in interacting with other synaptic molecules to mediate the formation and function of synaptic junctions. Lgi1 mainly associates with neuronal cell surfaces and is critical in synaptic stability and neurotransmitter release.

Biological function summary

Lgi1 influences synaptic transmission and plasticity. It forms a complex with the ADAM22 and ADAM23 proteins which are part of the disintegrin and metalloproteinase domain family. This complex regulates the activity and localization of ion channels involved in neuronal excitability influencing electrical signaling across neurons. These interactions are essential for maintaining proper neuronal communication and cognitive function.

Pathways

Several biological processes involve Lgi1 including the regulation of the voltage-gated potassium channel pathways and synaptic signaling pathways. Specifically Lgi1's interaction with ADAM22 facilitates the assembly of the postsynaptic density therefore contributing to synaptic strength. Moreover Lgi1 interacts with Kv1.1 an important partner in the modulation of ion channel activities that are central to neuronal signaling pathways.

Several conditions have a connection to Lgi1 especially autoimmune encephalitis and epilepsy. Autoimmune encephalitis often involves antibodies targeting Lgi1 affecting synaptic transmission and resulting in neurological symptoms. In epilepsy Lgi1 abnormalities potentially through disrupted interactions with related proteins like ADAM22 can lead to imbalances in neuronal excitability and trigger seizures. Understanding these connections helps in developing targeted therapies for these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.

See full target information LGI1

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重组Anti-Lgi1/EPT抗体[EPR9084] - BSA and Azide free