Anti-LexA DNA Binding Region抗体(ab174384)
Key features and details
- Rabbit polyclonal to LexA DNA Binding Region
- Suitable for: WB, IP, ChIP, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgG
概述
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产品名称
Anti-LexA DNA Binding Region抗体
参阅全部 LexA DNA Binding Region 一抗 -
描述
兔多克隆抗体to LexA DNA Binding Region -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ChIP, ICC/IFmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.
Database link: POA7C2 -
阳性对照
- E. coli whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab174384于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/3000. Predicted molecular weight: 22 kDa.
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| IP |
Use at an assay dependent concentration.
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| ChIP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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WB
1/1000 - 1/3000. Predicted molecular weight: 22 kDa. |
|
IP
Use at an assay dependent concentration. |
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ChIP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-terminal domain that contains three alpha-helices spanning residues. This characteristic of LexA protein has been used to make efficient baits for two-hybrid studies. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 1039346 Escherichia coli
- Entrez Gene: 948544 Escherichia coli
- Entrez Gene: 960080 Escherichia coli
- SwissProt: P0A7C2 Escherichia coli
- SwissProt: P0A7C3 Escherichia coli
- SwissProt: P0A7C4 Escherichia coli
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别名
- ExrA antibody
- Lex A antibody
- LexA antibody
see all
图片
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Western blot - Anti-LexA DNA Binding Region antibody (ab174384)All lanes : Anti-LexA DNA Binding Region antibody (ab174384) at 1/1000 dilution
Lane 1 : Marker
Lane 2 : E. coli whole cell lysate
Predicted band size: 22 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab174384 被引用在 5 文献中.
- Zlatkov N et al. Metabolic and Morphotypic Trade-Offs within the Eco-Evolutionary Dynamics of Escherichia coli. Microbiol Spectr 10:e0067822 (2022). PubMed: 36169422
- Hughes KJ et al. An RNA Repair Operon Regulated by Damaged tRNAs. Cell Rep 33:108527 (2020). PubMed: 33357439
- Kramarz K et al. The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal. Nat Commun 11:5643 (2020). PubMed: 33159083
- Sun Y et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
- Hishida T et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev 18:1886-97 (2004). WB ; Escherichia coli . PubMed: 15289460