重组Anti-Leukotriene B4 Receptor/BLT抗体[EPR7113] (ab131041)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)](https://www.abcam.cn/ps/products/131/ab131041/Images/ab131041-145258-anti-leukotriene-b4-receptor-antibody-epr7113-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7113] to Leukotriene B4 Receptor/BLT
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Leukotriene B4 Receptor/BLT抗体[EPR7113]
参阅全部 Leukotriene B4 Receptor/BLT 一抗 -
描述
兔单克隆抗体[EPR7113] to Leukotriene B4 Receptor/BLT -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human thymus, Human fetal lung and A549 cell lysates; Human breast carcinoma and tonsil tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.96 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7113 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131041于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response. -
组织特异性
Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1241 Human
- Omim: 601531 Human
- SwissProt: Q15722 Human
- Unigene: 567248 Human
- Unigene: 727320 Human
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别名
- BLT antibody
- BLT1 antibody
- BLTR antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)](https://www.abcam.cn/ps/products/131/ab131041/Images/ab131041-145259-anti-leukotriene-b4-receptor-antibody-epr7113-western-blot.jpg)
Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)All lanes : Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : Human fetal lung tissue lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)](https://www.abcam.cn/ps/products/131/ab131041/Images/ab131041-145257-anti-leukotriene-b4-receptor-antibody-epr7113-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)](https://www.abcam.cn/ps/products/131/ab131041/Images/ab131041-145256-anti-leukotriene-b4-receptor-antibody-epr7113.jpg)
OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041) -
![Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)](https://www.abcam.cn/ps/products/131/ab131041/Images/ab131041-5-benefits-of-recombinant-antibodies.png)
Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131041 被引用在 3 文献中.
- Su KK et al. Five-lipoxygenase-activating protein-mediated CYLD attenuation is a candidate driver in hepatic malignant lesion. Front Oncol 12:912881 (2022). PubMed: 35978827
- Wang L et al. Constitutive activation of the NEAT1/miR-22-3p/Ltb4r1 signaling pathway in mice with myocardial injury following acute myocardial infarction. Aging (Albany NY) 13:15307-15319 (2021). PubMed: 34081624
- Jeon WK et al. The proinflammatory LTB4/BLT1 signal axis confers resistance to TGF-ß1-induced growth inhibition by targeting Smad3 linker region. Oncotarget 6:41650-66 (2015). IHC . PubMed: 26497676