AB131041

Anti-Leukotriene B4 Receptor/BLT 抗体 [EPR7113]

Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal Leukotriene B4 Receptor/BLT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

查看别名

BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7, LTB4R, Leukotriene B4 receptor 1, LTB4-R 1, LTB4-R1, Chemoattractant receptor-like 1, G-protein coupled receptor 16, P2Y purinoceptor 7, P2Y7

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (AB131041)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (AB131041)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (AB131041)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling Leukotriene B4 Receptor/BLT with ab131041 antibody at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (AB131041)

All lanes:

Western blot - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (ab131041) at 1/10000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Human fetal lung tissue lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 38 kDa

false

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (AB131041)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] (AB131041)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-Leukotriene B4 Receptor/BLT antibody [EPR7113] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7113

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Leukotriene B4 Receptor often referred to as BLT or BLT1 plays a significant mechanical role in the immune response by binding to leukotriene B4 (LTB4) a potent chemoattractant. This receptor has a molecular mass of about 45 kDa. It expresses primarily on leukocytes including neutrophils macrophages and certain T cells. Furthermore BLT1 can also be observed in other tissues such as the spleen and liver where immune modulation has essential roles.

Biological function summary

The function of BLT1 involves mediating cell migration particularly during inflammatory responses. When LTB4 binds to BLT1 it activates intracellular signaling pathways that lead to chemotaxis and activation of immune cells. This receptor does not function in isolation; it forms part of receptor complexes on the cell surface to facilitate effective signal transduction. Although its name suggests specificity BLT1 can interact with other related lipid mediators amplifying its role in inflammation.

Pathways

Leukotriene B4 receptor BLT1 is integral to the arachidonic acid pathway and the GPCR signaling pathway. In the arachidonic acid pathway BLT1 contributes to the production of inflammatory mediators that recruit immune cells to sites of injury or infection. This receptor shares pathways with other GPCRs facilitating coordinated responses to external stimuli. It is closely related to protein kinase C (PKC) in the pathways which plays a role in signal transduction and cellular communication.

Leukotriene B4 receptor BLT1 has significant implications in chronic inflammatory diseases such as asthma and rheumatoid arthritis. Overactivation of BLT1 can result in excessive recruitment of immune cells exacerbating inflammation and tissue damage in these conditions. The receptor is also connected to COX-2 which is involved in the inflammation process linking BLT1 to the broader picture of inflammatory pathways in disease states. Understanding BLT1's role in these diseases helps identify potential therapeutic targets for managing inflammation-related pathologies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.

See full target information LTB4R

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:912881 PubMed35978827

2022

Five-lipoxygenase-activating protein-mediated CYLD attenuation is a candidate driver in hepatic malignant lesion.

Applications

Unspecified application

Species

Unspecified reactive species

Kun-Kai Su,Xue-Hua Zheng,Christian Bréchot,Xiao-Ping Zheng,Dan-Hua Zhu,Rong Huang,Yan-Hong Zhang,Jing-Jing Tao,Yi-Jia Lou,Lan-Juan Li

Aging 13:15307-15319 PubMed34081624

2021

Constitutive activation of the NEAT1/miR-22-3p/Ltb4r1 signaling pathway in mice with myocardial injury following acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Wang,Lu Wang,Qi Wang

Oncotarget 6:41650-66 PubMed26497676

2015

The proinflammatory LTB4/BLT1 signal axis confers resistance to TGF-β1-induced growth inhibition by targeting Smad3 linker region.

Applications

IHC

Species

Unspecified reactive species

Woo-Kwang Jeon,Jiyeon Choi,Seong Ji Park,Eun Ji Jo,Young K Lee,Seunghwan Lim,Jae-Hong Kim,John J Letterio,Fang Liu,Seong-Jin Kim,Byung-Chul Kim

View all publications

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Anti-Leukotriene B4 Receptor/BLT 抗体 [EPR7113]