Anti-Leptin Receptor抗体(ab5593)
Key features and details
- Rabbit polyclonal to Leptin Receptor
- Suitable for: IHC-Fr, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Leptin Receptor抗体
参阅全部 Leptin Receptor 一抗 -
描述
兔多克隆抗体to Leptin Receptor -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Leptin Receptor aa 577-594 (extracellular).
Sequence:KEIQWKTHEVFDAKSKSA
(Peptide available asab5837) -
阳性对照
- WB: Mouse lung tissue extract.
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常规说明
As per Uniprot database (P48357) OB receptor has 5 isoforms and is highly glycosylated, this means the observed band size will be different than predicted and multiple bands are expected depending upon the isoforms expressed in sample types.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
纯化说明
No concentration is available. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| WB | (4) |
1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837).
ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R.
|
| IP |
Use at an assay dependent concentration. PubMed: 21151569
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
1/2000. Detects a band of approximately 100, 125 kDa.Can be blocked with Leptin Receptor peptide (ab5837). ab5593 detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R.
|
|
IP
Use at an assay dependent concentration. PubMed: 21151569 |
靶标
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功能
Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway. -
组织特异性
Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus. -
序列相似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 4 fibronectin type-III domains.
Contains 1 Ig-like (immunoglobulin-like) domain. -
结构域
The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻译后修饰
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3953 Human
- Entrez Gene: 16847 Mouse
- Entrez Gene: 24536 Rat
- Omim: 601007 Human
- SwissProt: P48357 Human
- SwissProt: P48356 Mouse
- SwissProt: Q62959 Rat
- Unigene: 23581 Human
see all -
别名
- CD 295 antibody
- CD295 antibody
- CD295 antigen antibody
see all
图片
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Western blot - Anti-Leptin Receptor antibody (ab5593)Anti-Leptin Receptor antibody (ab5593) at 1/2000 dilution + Mouse lung tissue lysate
Western blot detction of both short and long forms of Leptin (OB-R).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab5593 被引用在 35 文献中.
- Tan R et al. Leptin Promotes the Proliferation and Neuronal Differentiation of Neural Stem Cells through the Cooperative Action of MAPK/ERK1/2, JAK2/STAT3 and PI3K/AKT Signaling Pathways. Int J Mol Sci 24:N/A (2023). PubMed: 37894835
- Qiu W et al. Xiaoyaosan ameliorates depressive-like behavior and susceptibility to glucose intolerance in rat: involvement of LepR-STAT3/PI3K pathway in hypothalamic arcuate nucleus. BMC Complement Med Ther 23:116 (2023). PubMed: 37046230
- Balatskiy A et al. Immature Vascular Smooth Muscle Cells in Healthy Murine Arteries and Atherosclerotic Plaques: Localization and Activity. Int J Mol Sci 23:N/A (2022). PubMed: 35163667
- Kim J & Lee M RMR-Related DNAJC6 Expression Suppresses Adipogenesis in 3T3-L1 Cells. Cells 11:N/A (2022). PubMed: 35456010
- Ramírez-Plascencia OD et al. Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats. Front Neurosci 16:907508 (2022). PubMed: 35937866