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Anti-Leptin Receptor抗体(ab50424)

Submit a review Q&A (2)References (1)
Western blot - Anti-Leptin Receptor antibody (ab50424)
  • Flow Cytometry - Anti-Leptin Receptor antibody (ab50424)

Key features and details

  • Goat polyclonal to Leptin Receptor
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Leptin Receptor抗体
    参阅全部 Leptin Receptor 一抗

  • 描述

    山羊多克隆抗体to Leptin Receptor

  • 宿主

    Goat

  • 特异性

    This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1) of Leptin Receptor.

  • 经测试应用

    适用于: IHC-P, Flow Cyt, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Dog

  • 免疫原

    Synthetic peptide corresponding to Human Leptin Receptor aa 829-841 (internal sequence).
    Sequence:

    C-TQDDIEKHQSDAG


    Database link: P48357
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Human Cerebellum, Mouse Foetal Brain and Rat Brain lysate. Flow Cyt: K562 cells

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab50424于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).

1 hour primary incubation is recommended for this product.
说明
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 132 kDa).

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.

  • 组织特异性

    Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.

  • 序列相似性

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 Ig-like (immunoglobulin-like) domain.

  • 结构域

    The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • 翻译后修饰

    On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation and, for interaction with SOCS3 (By similarity). Phosphorylation on Tyr-1141 is required for STAT3 binding/activation.

  • 细胞定位

    Secreted and Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CD 295 antibody
    • CD295 antibody
    • CD295 antigen antibody
    • Db antibody
    • Fa antibody
    • HuB219 antibody
    • LEP R antibody
    • LEP-R antibody
    • LEPR antibody
    • LEPR_HUMAN antibody
    • LEPRD antibody
    • Leptin receptor antibody
    • Leptin receptor fatty antibody
    • Leptin receptor gene related protein antibody
    • Leptin receptor precursor antibody
    • Leptin receptor precursor antibody
    • OB R gene related protein antibody
    • OB receptor antibody
    • OB-R antibody
    • OB-RGRP antibody
    • obl antibody
    • Obr antibody
    see all

图片

  • Western blot - Anti-Leptin Receptor antibody (ab50424)
    Western blot - Anti-Leptin Receptor antibody (ab50424)
    Lane 1 : Anti-Leptin Receptor antibody (ab50424) at 1.75 µg/ml
    Lanes 2-3 : Anti-Leptin Receptor antibody (ab50424) at 1.5 µg/ml

    Lane 1 : Human Cerebellum tissue lysate
    Lane 2 : Mouse Foetal Brain tissue lysate
    Lane 3 : Rat Brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 132 kDa



    Lysate in RIPA buffer. Detected by chemiluminescence.

  • Flow Cytometry - Anti-Leptin Receptor antibody (ab50424)
    Flow Cytometry - Anti-Leptin Receptor antibody (ab50424)
    Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

文献 (1)

发表研究结果有使用 ab50424？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab50424 被引用在 1 文献中.

  • Maranesi M  et al. Leptin System in Obese Dog Skin: A Pilot Study. Animals (Basel) 10:N/A (2020). PubMed: 33316917

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Hello,

Weneedtwo different types ofanti-leptin antibodies for human milk samples, that each ones recognize different epitopes; and a third antibody anti-Ig against the animal where the anti-leptin antibodies were produced. Wich of yours products do you recomend me? We'd like to know exactly the price of each product, the quantity/volume,how long will it take to arrive to Mallorca and if it's only for investigation because we want to introduce it in a commercial instrument.

I'm waiting for your answer. Thanks

Read More
Answer

Thank you for contacting us and your interest in our products.

We have a number of different antibodies directed against the human Leptin protein. The ideal choices for your experiment would depend on what application you are hoping to use.

We have several antibodies which are able to detect different epitopes from the Leptinprotein such as ab50424 which is a goat polyclonal raised againsta synthetic peptide derived from the residues 829-841 of human Leptin. This antibody has been used in ELISA and western blotting.

The antibodyab20101 (mouse monoclonal)has been raised against the peptide taken from residues 131-145 of human Leptin. This antibody has been used in sandwich ELISA, Western blotting and IHC. The antibody ab20101 was used as an ELISA sandwich pair with the rabbit polyclonal ab7208 which was raised against the full length human Leptin protein. The standard curve was performed using the recombinantly expressed human Leptinprotein (ab51273).

In order to advise which secondary antibody (anti-IgG of host animal) would be suitable, I would need to know which conjugate you would like to use in your assay. It would also be helpful to know the application in which you wish to use the antibodies. If you could provide me with this information I will be able to advise you further. If you could let me knowwhich antibodies you'd be interested in I can also let you know the delivery time and how much antibody would be provided (alternatively this information isavailable on the datasheet of each product).

All of our products are for researchpurposes only and are not intended for diagnostic, therapeutic or in vivo use.

I hope this information has been of some help. If you require any further information please do not hesitate to contact us again.

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Question

Does this product contain the isoform b?

Read More
Answer

Thank you for your enquiry. I can confirm that the immunizing peptide is in all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1). NCBI calls them isoforms 1, 2, and 3 resp, but the alternative named isoforms are CRA_b, CRA_c and CRA_d, and also contain the immunizing peptide. Therefore, this product contains the isoform b you are looking for.

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