重组Anti-Leptin抗体[EPR16848-34] (ab181298)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16848-34] to Leptin
- Suitable for: Blocking
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Leptin抗体[EPR16848-34]
参阅全部 Leptin 一抗 -
描述
兔单克隆抗体[EPR16848-34] to Leptin -
宿主
Rabbit -
经测试应用
适用于: Blockingmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Blocking Assay: HEK-293 cells co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR16848-34 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Blocking |
Use at an assay dependent concentration.
(50 µg/ml-100 µg/ml). |
说明 |
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Blocking
Use at an assay dependent concentration. (50 µg/ml-100 µg/ml). |
靶标
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功能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
疾病相关
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
序列相似性
Belongs to the leptin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3952 Human
- Entrez Gene: 16846 Mouse
- Omim: 164160 Human
- SwissProt: P41159 Human
- SwissProt: P41160 Mouse
- Unigene: 194236 Human
- Unigene: 277072 Mouse
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别名
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
图片
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HEK-293 cells were co-transfected with a plasmid encoding the full-length mouse leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.
ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37℃ before adding to transfected cells.
Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).
The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.
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HEK-293 cells were co-transfected with a plasmid encoding full length human leptin receptor, pRL-CMV and pGL4.47[luc2P/SIE/Hygro] Vectors. Dual-Luciferase® Reporter Assay was used to test the leptin signaling.
ab181298 (0, 50, 100 µg/ml) was incubated with recombinant mouse leptin in a total of 500ul culture medium at the final concentration of 100, 50, 25, and 12.5 ng/ml for 2h at 37℃ before adding to transfected cells.
Leptin without incubating with ab181298 as the positive control (green) shows the maximal signal of the leptin and leptin receptor interaction, whereas the base line signal (without adding leptin and ab181298 to the system) serves as the negative control (purple).
The data demonstrates that the interaction of leptin and leptin receptor can be blocked by ab181298 resulting in decreased florescence signals.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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