AB3583

Anti-Leptin抗体

Name: Leptin抗体 (ab3583)| Abcam中文官网
Brand: Abcam Limited
SKU: AB3583
Price: 4996 CNY
Availability: InStock
Rating: 2 (3 reviews)

Anti-Leptin antibody

(3 Reviews)

(34 Publications)

Rabbit Polyclonal Leptin antibody. Suitable for ICC, Flow Cyt, IHC-Fr and reacts with Human, Mouse, Sheep samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Mouse Lep aa 1-50.

查看别名

Ob, Lep, Leptin, Obesity factor

5 Images

AbReview31591****

Immunocytochemistry - Anti-Leptin antibody (AB3583)

ab3583 staining Leptin in Human mesenchyml stem cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% horse serum) for 1 hour at 22°C. A FITC-conjugated Mouse anti-rabbit IgG1 monoclonal was used as the secondary antibody (1/100).

This image is courtesy of an Abreview submitted by Steven Johnstone

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Immunocytochemistry - Anti-Leptin antibody (AB3583)

ICC image of ab3583 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3583, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Supplier Data

Immunocytochemistry - Anti-Leptin antibody (AB3583)

ab3583 staining Leptin in 3T3-L1 cells by ICC (Immunocytochemistry). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody (1/2000) (Panel a). Nuclei were stained with DAPI (Panel b). F-actin stained with Rhodamine Phalloidin (panel c). Merged images shown in panel d.

Flow Cytometry - Anti-Leptin antibody (AB3583)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Leptin antibody (AB3583)

ab3583 staining Leptin in 3T3-L1 cells by Flow Cytometry. The sample was incubated with the primary antibody (3ug/ml in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor 488®-conjugated Goat anti-rabbit (1/400) was used as the secondary antibody. Red histogram represents ab3583, pink histogram represents isotype control, purple histogram represents unstained control, green histogram represents no primary antibody control

Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (AB3583)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (AB3583)

Immunohistochemical staining of Leptin in Sheep brain using ab3583.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Sheep, Human

应用

Flow Cyt, ICC, IHC-Fr

applications

免疫原

Synthetic Peptide within Mouse Lep aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P41160

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3-5 µL for 10^6 Cells", "FlowCyt-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Goat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Horse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50 - 1/200", "IHCFr-species-notes": "" }, "Turkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Leptin also known as the OB protein is a hormone with a molecular weight of approximately 16 kDa. Primarily it is produced by adipocytes in white adipose tissue and to a lesser extent in the placenta stomach and other tissues. This protein plays a significant role in energy homeostasis by conveying information to the central nervous system regarding fat storage. In scientific studies leptin is often measured using methods like the leptin ELISA kit or mouse leptin assay to monitor its levels and function.

Biological function summary

Leptin regulates food intake and energy expenditure by binding to its receptors in the hypothalamus. It is an important part of the feedback loop to manage body weight and appetite. Leptin is not part of a larger protein complex and acts directly on its target tissues. By signal transduction pathways it influences cellular processes such as metabolism and even impacts fertility and immune responses.

Pathways

Leptin is involved in the neuroendocrine signaling pathway and the MAPK/ERK pathway. In the neuroendocrine system leptin interacts with proteins like proopiomelanocortin (POMC) and neuropeptide Y (NPY) to manage appetite and energy use. Additionally it activates the JAK/STAT pathway when binding to its receptor which influences gene expression involved in metabolism and inflammation.

Leptin is linked to obesity and type 2 diabetes. Leptin deficiencies or resistance can lead to metabolic disorders as the hormone's signaling is disrupted affecting energy balance and storage. Its connection to adiponectin another hormone derived from fat tissue is critical in understanding these conditions; while leptin promotes inflammation adiponectin has anti-inflammatory effects. Exploring these proteins' roles and their balance offers insights into treatments for obesity-related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways (PubMed : 11373681, PubMed : 12594516, PubMed : 15899045, PubMed : 16825198, PubMed : 20620997). In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity (By similarity) (PubMed : 10660043, PubMed : 12594516, PubMed : 25060689, PubMed : 25383904, PubMed : 8589726, PubMed : 9732873). In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides (By similarity) (PubMed : 11373681, PubMed : 20620997). In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways (By similarity) (PubMed : 16825198, PubMed : 20620997). May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression (By similarity). Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) (PubMed : 16825198). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells (Probable). Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 which promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38 (PubMed : 15899045). In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses (By similarity). Increases CD4(+)CD25(-) T cells proliferation and reduces autophagy during TCR (T cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation (PubMed : 25060689).

See full target information Lep

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文献 (34)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 13: PubMed36836789

2023

Localization of FGF21 Protein and Lipid Metabolism-Related Genes in Camels.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Shuqin Zhao,Wangdong Zhang,Huaping Tang,Meilin Yan,Fang Yong,Xu Bai,Xiaochun Wu,Yong Zhang,Quanwei Zhang

PeerJ 10:e13982 PubMed36187750

2022

Presence of leptin and its receptor in the ram reproductive system and effect of leptin on sperm quality.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Gao,Guodong Zhao,Yukun Song,Aerman Haire,Ailing Yang,Xi Zhao,Abulizi Wusiman

Journal of experimental & clinical cancer research : CR 41:215 PubMed35778755

2022

Aberrant miR-874-3p/leptin/EGFR/c-Myc signaling contributes to nasopharyngeal carcinoma pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Dean Luo,Hsin-Ting Tsai,Chung-Feng Hwang,Tai-Jan Chiu,Shau-Hsuan Li,Ya-Ling Hsu,Chang-Chun Hsiao,Chang-Han Chen

Analytical cellular pathology (Amsterdam) 2022:9994906 PubMed35111566

2022

Gallbladder Cancer Cell-Derived Exosome-Mediated Transfer of Leptin Promotes Cell Invasion and Migration by Modulating STAT3-Mediated M2 Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Songling Zhao,Yunxia Liu,Linhai He,Yuehua Li,Ke Lin,Qiang Kang,Lixin Liu,Hao Zou

Journal of the American Heart Association 10:e021369 PubMed34482701

2021

Pericardial Adipose Tissue-Derived Leptin Promotes Myocardial Apoptosis in High-Fat Diet-Induced Obese Rats Through Janus Kinase 2/Reactive Oxygen Species/Na+/K+-ATPase Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Wang,Chaodi Luo,Danjun Zhu,Yan Song,Lifei Cao,Hui Luan,Lan Gao,Shuping Zheng,Hao Li,Gang Tian

Open medicine (Warsaw, Poland) 16:1215-1227 PubMed34514168

2021

miR-519d downregulates LEP expression to inhibit preeclampsia development.

Applications

Unspecified application

Species

Unspecified reactive species

Hairui Cai,Dongmei Li,Jun Wu,Chunbo Shi

Scientific reports 11:17001 PubMed34417537

2021

Systemic versus local adipokine expression differs in a combined obesity and osteoarthritis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Lisa Hülser,Yubin Luo,Klaus Frommer,Rebecca Hasseli,Kernt Köhler,Magnus Diller,Lina Van Nie,Christoph Rummel,Martin Roderfeld,Elke Roeb,Georg Schett,Aline Bozec,Ulf Müller-Ladner,Elena Neumann

Biochimica et biophysica acta. General subjects 1865:129973 PubMed34352342

2021

Cladosporols A and B, two natural peroxisome proliferator-activated receptor gamma (PPARγ) agonists, inhibit adipogenesis in 3T3-L1 preadipocytes and cause a conditioned-culture-medium-dependent arrest of HT-29 cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Rapuano,Pamela Ziccardi,Valentina Cioffi,Sabrina Dallavalle,Salvatore Moricca,Angelo Lupo

Experimental and therapeutic medicine 22:1023 PubMed34373709

2021

mRNA expression level of , , , and modulates 5-fluorouracil resistance in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Rongmu Xia,Chenmeng Li,Xiaocong Chen,Xuemin Cai,Wengang Li

Frontiers in veterinary science 8:625147 PubMed33644151

2021

Serum and Tissue Expression Levels of Leptin and Leptin Receptor Are Putative Markers of Specific Feline Mammary Carcinoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Gameiro,Catarina Nascimento,Ana Catarina Urbano,Jorge Correia,Fernando Ferreira

View all publications

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Anti-Leptin抗体

Anti-Leptin antibody

Immunocytochemistry - Anti-Leptin antibody (AB3583)

Immunocytochemistry - Anti-Leptin antibody (AB3583)

Immunocytochemistry - Anti-Leptin antibody (AB3583)

Flow Cytometry - Anti-Leptin antibody (AB3583)

Immunohistochemistry (Frozen sections) - Anti-Leptin antibody (AB3583)