Anti-LEPREL1抗体(ab222823)
Key features and details
- Rabbit polyclonal to LEPREL1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-LEPREL1抗体 -
描述
兔多克隆抗体to LEPREL1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human LEPREL1 aa 301-528.
Database link: Q8IVL5 -
阳性对照
- WB: Mouse kidney and lung lysates. IHC-P: Human liver and lung tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222823于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/200.
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| WB |
1/2000 - 1/5000. Detects a band of approximately 81, 61 kDa (predicted molecular weight: 81 kDa).
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| 说明 |
|---|
|
IHC-P
1/20 - 1/200. |
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WB
1/2000 - 1/5000. Detects a band of approximately 81, 61 kDa (predicted molecular weight: 81 kDa). |
靶标
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功能
Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V. -
组织特异性
Expressed in heart, placenta, lung, liver, skeletal muscle and kidney. Expression localized to the epithelia of bile ducts and to the sacroplasm of heart muscle and skeletal muscle. In the pancreas, localized to a subpopulation of Langerhans islet cells and in the salivary gland, expressed in acinar cells. -
序列相似性
Belongs to the leprecan family.
Contains 1 Fe2OG dioxygenase domain.
Contains 4 TPR repeats. -
细胞定位
Endoplasmic reticulum. Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 55214 Human
- Entrez Gene: 210530 Mouse
- Omim: 610341 Human
- SwissProt: Q8IVL5 Human
- SwissProt: Q8CG71 Mouse
- Unigene: 374191 Human
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别名
- 4832416N06 antibody
- AW553532 antibody
- Leprecan like 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (ab222823)
Paraffin-embedded human liver tissue stained for LEPREL1 with ab222823 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (ab222823)Paraffin-embedded human lung tissue stained for LEPREL1 with ab222823 at 1/100 dilution in immunohistochemical analysis.
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Western blot - Anti-LEPREL1 antibody (ab222823)All lanes : Anti-LEPREL1 antibody (ab222823) at 1/2000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse kidney lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 81 kDa
Observed band size: 61,81 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab222823 被引用在 1 文献中.
- You YH et al. MicroRNA-153 promotes brain-derived neurotrophic factor and hippocampal neuron proliferation to alleviate autism symptoms through inhibition of JAK-STAT pathway by LEPR. Biosci Rep 39:N/A (2019). PubMed: 30975733