AB232627

重组Anti-Legumain抗体[EPR14718] - BSA and Azide free

Anti-Legumain antibody [EPR14718] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Legumain antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

PRSC1, LGMN, Legumain, Asparaginyl endopeptidase, AEP

1 Images

Lab

Western blot - Anti-Legumain antibody [EPR14718] - BSA and Azide free (AB232627)

Actual band at : 36kDa, 46kDa, 56kDa.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183028).

All lanes:

Western blot - Anti-Legumain antibody [EPR14718] (<a href='/products/primary-antibodies/legumain-antibody-epr14718-ab183028'>ab183028</a>) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

JAR (human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Predicted band size: 49 kDa

false

Exposure time: 3min

不同偶联物与剂型 (1)

Unconjugated

Anti-Legumain antibody [EPR14718]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14718

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab232627 is the carrier-free version of ab183028.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Legumain also known as asparaginyl endopeptidase is an enzyme that plays a mechanical role as a protease. This enzyme has a molecular mass of approximately 56 kDa and it functions by catalyzing the hydrolysis of asparagine residues. Legumain is expressed in various tissues with higher levels observed in kidneys placenta and liver. Its proteolytic activity is essential for the breakdown of intracellular proteins within lysosomes and endosomal compartments.

Biological function summary

Legumain contributes to protein degradation and antigen processing. It does not operate alone as it is part of larger protein complexes which enhance its stability and function. Through its role in proteolysis legumain influences cellular processes like maturation of endolysosomal enzymes and processing of proenzymes. Additionally it assists in activating other proteases signifying its critical position in maintaining cellular proteostasis.

Pathways

Legumain participates in the regulation of immune responses and cellular homeostasis. It plays a significant role in the lysosomal pathway where it partners with cathepsins to assist in the degradation of proteins. Additionally legumain influences the MHC class II pathway by contributing to the cleavage of invariant chain aiding antigen presentation. Its interaction with cathepsins and the impact on antigen processing indicates its necessity in immune system functionality.

Legumain has been linked to disorders such as cancer and neurodegeneration. In cancer elevated legumain levels correlate with tumor invasiveness and metastasis especially in association with proteins like matrix metalloproteinases. Meanwhile in neurodegenerative conditions its dysregulation may contribute to abnormal protein accumulation and synaptic dysfunction. Understanding legumain's role and interactions can guide therapeutic strategies for these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed : 23776206). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed : 23776206). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (PubMed : 9872320). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules (By similarity). Required for normal degradation of internalized EGFR (By similarity). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity).

See full target information LGMN

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 5:572-578 PubMed37037945

2023

A proteo-transcriptomic map of non-alcoholic fatty liver disease signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Olivier Govaere,Megan Hasoon,Leigh Alexander,Simon Cockell,Dina Tiniakos,Mattias Ekstedt,Jörn M Schattenberg,Jerome Boursier,Elisabetta Bugianesi,Vlad Ratziu,Ann K Daly,Quentin M Anstee

View all publications

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-Legumain抗体[EPR14718] - BSA and Azide free