Anti-LECT2抗体[OTI2A11] (ab119429)
![Western blot - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132770-anti-lect2-antibody-oti2a11-western-blot.jpg "Western blot - Anti-LECT2 antibody [OTI2A11] (ab119429)")
Key features and details
- Mouse monoclonal [OTI2A11] to LECT2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-LECT2抗体[OTI2A11]
参阅全部 LECT2 一抗 -
描述
小鼠单克隆抗体[OTI2A11] to LECT2 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human LECT2 aa 1-200. Produced in E.coli (NP_002293). aa 1-18 are the signal peptide, immunogen is to the full length mature chain.
Database link: O14960 -
阳性对照
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY LECT2 cDNA. IHC-P: Human breast, Kidney, Lung carcinoma, Ovary adenocarcinoma, Pancreas, Endometrium adenocarcinoma and Tonsil tissues.
-
常规说明
The clone number has been updated from 2A11 to OTI2A11, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI2A11 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119429于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 16 kDa.
|
|
| IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500. Predicted molecular weight: 16 kDa. |
|
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. -
组织特异性
Highly expressed in adult and fetal liver and weakly in testis. Not expressed in bone marrow. -
序列相似性
Belongs to the LECT2/MIM-1 family. -
细胞定位
Cytoplasm. Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 3950 Human
- Omim: 602882 Human
- SwissProt: O14960 Human
- Unigene: 512580 Human
-
别名
- chm II antibody
- chm2 antibody
- Chondromodulin II antibody
see all
图片
-
Western blot - Anti-LECT2 antibody [OTI2A11] (ab119429)All lanes : Anti-LECT2 antibody [OTI2A11] (ab119429) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY LECT2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 16 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132769-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human breast tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132768-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human kidney tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132767-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human lung carcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132766-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human ovary adenocarcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132765-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human pancreas tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132764-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human endometrium adenocarcinoma tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)](https://www.abcam.cn/ps/products/119/ab119429/Images/ab119429-132763-anti-lect2-antibody-oti2a11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LECT2 antibody [OTI2A11] (ab119429)Paraffin-embedded human tonsil tissue stained for LECT2 using ab119429 at 1/150 dilution in immunohistochemical analysis.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab119429 被引用在 2 文献中.
- Shirasaki T et al. Leukocyte cell-derived chemotaxin 2 is an antiviral regulator acting through the proto-oncogene MET. Nat Commun 13:3176 (2022). PubMed: 35676290
- Xu M et al. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis. Cell 178:1478-1492.e20 (2019). PubMed: 31474362