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AB167416

重组Anti-LDLC抗体[EPR10896]

Anti-LDLC antibody [EPR10896]

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(1 Publication)

Rabbit Recombinant Monoclonal LDLC antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

LDLC, COG2, Conserved oligomeric Golgi complex subunit 2, COG complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling LDLC with ab167416 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-LDLC antibody [EPR10896] (AB167416)
  • WB

Unknown

Western blot - Anti-LDLC antibody [EPR10896] (AB167416)

All lanes:

Western blot - Anti-LDLC antibody [EPR10896] (ab167416) at 1/1000 dilution

Lane 1:

Fetal lung tissue lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 83 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LDLC with ab167416 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10896

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LDLC also known as Low-Density Lipoprotein Cholesterol is a significant component of the plasma lipid transport system. It plays a mechanical role in transporting cholesterol to peripheral tissues. The molecular mass of LDLC varies depending on the size of the lipoprotein particles typically ranging from 2000 to 3000 kDa. LDLC prominently circulates in the blood and is widely expressed throughout the body particularly in liver and intestines where it is synthesized and secreted.
Biological function summary

LDLC serves as the primary cholesterol carrier and is essential for cellular membrane synthesis and maintenance. It forms a complex with apolipoproteins such as ApoB-100 which facilitate interaction with LDL receptors on cell surfaces. This interaction enables the uptake of cholesterol for use in intracellular processes. LDL particles derive from very low-density lipoproteins (VLDL) following triglyceride depletion emphasizing their role in lipid metabolism.

Pathways

The cholesterol transport and lipid metabolism pathways involve LDLC as a core component. One important pathway is the mevalonate pathway where it links to biosynthesis of cholesterol steroids and isoprenoids. The protein SREBP (Sterol Regulatory Element Binding Protein) regulates the expression of LDL receptors impacting cholesterol uptake efficiency. Another relevant pathway is the reverse cholesterol transport pathway involving ApoA-I where excess cholesterol from tissues returns to the liver for excretion.

LDLC is strongly associated with cardiovascular diseases especially atherosclerosis. Elevated levels of LDLC contribute to plaque formation in arterial walls leading to heart diseases. Familial hypercholesterolemia a genetic disorder also links to increased LDLC levels due to defective LDL receptors caused by mutations in the LDLR gene. Apolipoprotein E (ApoE) polymorphisms further affect the clearance rate of LDLC influencing susceptibility to these conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required for normal Golgi morphology and function.
See full target information COG2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 39: PubMed30988160

2019

Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.

Applications

Unspecified application

Species

Unspecified reactive species

David McClellan,Mattie J Casey,Diana Bareyan,Helena Lucente,Christopher Ours,Matthew Velinder,Jason Singer,Mehraju Din Lone,Wenxiang Sun,Yunuen Coria,Clinton C Mason,Michael E Engel
View all publications
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