重组Anti-LDL Receptor抗体[EP1553Y] (ab52818)

Anti-LDLR antibody [EP1553Y] (ab52818) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52818 was shown to bind specifically to LDLR. A band was observed at 130/160 kDa in wild-type HeLa cell lysates with no signal observed at this size in LDLR knockout cell line ab273838 (knockout cell lysate ab273792). To generate this image, wild-type and LDLR knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

Blocking buffer: 5% NFDM/TBST

Diluting buffer: 5% NFDM/TBST

Blocking buffer: 5% NFDM/TBST

Diltuion buffer: 5% NFDM/TBST

Immunohistochemical analysis of paraffin-embedded human liver sections labeling LDL Receptor with purified ab52818 at dilution of 1:500. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Blocking and diluting buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with the literature (PMID: 15199428, PMID: 8349823, PMID: 10906332, PMID: 24918045).

Blocking buffer: 5% NFDM/TBST

Diltuion buffer: 5% NFDM/TBST

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling LDL Receptor with ab52818 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counterstained with hematoxylin.