Anti-LDH-C抗体[EP1746Y] (ab52747)
![Western blot - Anti-LDH-C antibody [EP1746Y] (ab52747)](https://www.abcam.cn/ps/products/52/ab52747/Images/ab52747-65171-anti-ldh-c-antibody-ep1746y-western-blot.jpg "Western blot - Anti-LDH-C antibody [EP1746Y] (ab52747)")
Key features and details
- Rabbit monoclonal [EP1746Y] to LDH-C
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-LDH-C抗体[EP1746Y]
参阅全部 LDH-C 一抗 -
描述
兔单克隆抗体[EP1746Y] to LDH-C -
宿主
Rabbit -
特异性
This antibody reacts with Lactate Dehydrogenase C / LDH-C. The immunogen sequence is 100% identical to Lactate Dehydrogenase C / LDH-C, but there is the possibility of cross-reactivity for lactate dehydrogenase A and lactate dehydrogenase B at 69% and 82%, respectively.
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经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra)more details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human LDH-C aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P07864 -
阳性对照
- WB: Human testis lysate. IHC-P: Human testis carcinoma tissue. Flow Cyt (intra): SK-BR-3 cells
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1746Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52747于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/40.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| 说明 |
|---|
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WB
1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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靶标
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功能
Possible role in sperm motility. -
通路
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
序列相似性
Belongs to the LDH/MDH superfamily. LDH family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 3948 Human
- Omim: 150150 Human
- SwissProt: P07864 Human
- Unigene: 654377 Human
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别名
- Cancer/testis antigen 32 antibody
- CT32 antibody
- EC 1.1.1.27 antibody
see all
图片
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Western blot - Anti-LDH-C antibody [EP1746Y] (ab52747)Anti-LDH-C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] (ab52747)](https://www.abcam.cn/ps/products/52/ab52747/Images/ab52747-65170-anti-ldh-c-antibody-ep1746y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDH-C antibody [EP1746Y] (ab52747)Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] (ab52747)](https://www.abcam.cn/ps/products/52/ab52747/Images/ab52747-3-anti-ldh-c-antibody-ep1746y-flowcytometry-skbr3-human.jpg)
Flow Cytometry (Intracellular) - Anti-LDH-C antibody [EP1746Y] (ab52747)Flow Cytometry analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell) cells labeling LDH-C with ab52747 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab52747 被引用在 9 文献中.
- Tan H et al. Identification of human LDHC4 as a potential target for anticancer drug discovery. Acta Pharm Sin B 12:2348-2357 (2022). PubMed: 35646544
- Peng W et al. Cancer-Testis Antigen LDH-C4 in Tissue, Serum, and Serum-Derived Exosomes Serves as a Promising Biomarker in Lung Adenocarcinoma. Front Oncol 12:912624 (2022). PubMed: 35814471
- Chen L et al. Cancer/testis antigen LDHC promotes proliferation and metastasis by activating the PI3K/Akt/GSK-3ß-signaling pathway and the in lung adenocarcinoma. Exp Cell Res 398:112414 (2021). PubMed: 33301764
- Thomas R et al. Identification of two HLA-A*0201 immunogenic epitopes of lactate dehydrogenase C (LDHC): potential novel targets for cancer immunotherapy. Cancer Immunol Immunother 69:449-463 (2020). PubMed: 31932876
- Cui Z et al. Cancer-testis antigen lactate dehydrogenase C4 in hepatocellular carcinoma: a promising biomarker for early diagnosis, efficacy evaluation and prognosis prediction. Aging (Albany NY) 12:19455-19467 (2020). PubMed: 33035196
- Muranaka F et al. A Comparative Immunohistochemical Study of Anal Canal Epithelium in Humans and Swine, Focusing on the Anal Transitional Zone Epithelium and the Anal Glands. Anat Rec (Hoboken) N/A:N/A (2017). PubMed: 29232767
- Kong L et al. Expression of lactate dehydrogenase C in MDA-MB-231 cells and its role in tumor invasion and migration. Mol Med Rep 13:3533-8 (2016). PubMed: 26935238
- Rolland AD et al. Identification of genital tract markers in the human seminal plasma using an integrative genomics approach. Hum Reprod 28:199-209 (2013). PubMed: 23024119
- Wyns C et al. Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Hum Reprod 23:2402-14 (2008). IHC-P ; Human . PubMed: 18664476