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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-LDB2抗体(ab3627)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Western blot - Anti-LDB2 antibody (ab3627)

    Key features and details

    • Rabbit polyclonal to LDB2
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-LDB2抗体
    • 描述

      兔多克隆抗体to LDB2
    • 宿主

      Rabbit
    • 特异性

      This antibody detects a single clean band representing LDB2 at 42kD in human kidney and mouse spleen.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Human
      预测可用于: Chicken
    • 免疫原

      Synthetic peptide:

      SKESYHNSSITVDC

      conjugated to KLH, corresponding to amino acids 107-120 of Human LDB2.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3627于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    说明
    应用说明
    ICC/IF: Use at an assay dependent dilution (PMID 19675209).
    WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42.7 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    靶标

    • 功能

      Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors.
    • 序列相似性

      Belongs to the LDB family.
    • 翻译后修饰

      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome.
    • 细胞定位

      Nucleus.
    • Target information above from: UniProt accession O43679 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 395631 Chicken
      • Entrez Gene: 9079 Human
      • Entrez Gene: 16826 Mouse
      • Omim: 603450 Human
      • SwissProt: Q9W676 Chicken
      • SwissProt: O43679 Human
      • SwissProt: O55203 Mouse
      • Unigene: 714330 Human
      • Unigene: 25785 Mouse
      see all
    • 别名

      • Carboxyl terminal LIM domain binding protein 1 antibody
      • Carboxyl terminal LIM domain protein 1 antibody
      • Carboxyl-terminal LIM domain-binding protein 1 antibody
      • CLIM 1 antibody
      • CLIM-1 antibody
      • CLIM1 antibody
      • LDB 1 antibody
      • LDB 2 antibody
      • LDB-2 antibody
      • LDB1 antibody
      • LDB2 antibody
      • LDB2_HUMAN antibody
      • LIM binding domain 2 antibody
      • LIM domain binding 2 antibody
      • LIM domain binding factor 2 antibody
      • LIM domain binding factor CLIM 1 antibody
      • LIM domain binding factor CLIM1 antibody
      • LIM domain binding protein 2 antibody
      • LIM domain-binding factor CLIM1 antibody
      • LIM domain-binding protein 2 antibody
      • OTTHUMP00000158632 antibody
      • OTTHUMP00000217823 antibody
      • OTTHUMP00000217824 antibody
      • OTTHUMP00000217825 antibody
      • OTTHUMP00000217827 antibody
      see all

    图片

    • Western blot - Anti-LDB2 antibody (ab3627)
      Western blot - Anti-LDB2 antibody (ab3627)

      Western blot using ab3627 at 1/500.

      Lane 1: Human Kidney
      Lane 2: Mouse Spleen

      Lysates at 18µg per lane.
      Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
      ab7090 (1/5000).
      Exposure time: 1 minute.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab3627?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3627 被引用在 1 文献中.

    • Storbeck CJ  et al. The Ldb1 and Ldb2 transcriptional cofactors interact with the Ste20-like kinase SLK and regulate cell migration. Mol Biol Cell 20:4174-82 (2009). ICC/IF ; Mouse . PubMed: 19675209

    客户评价及客户问答

    Show All 评价 Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-LDB2 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic spinal cord)
    Permeabilization
    Yes - 0.1%Triton X-100
    Specification
    Embryonic spinal cord
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Frederic Clotman

    Verified customer

    提交于 Aug 20 2018

    Question

    Do you have the peptide available for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2004

    Answer

    Thank you for your enquiry. Unfortunately the peptide is not available for purchasing. If you have any further questions, please let us know.

    Read More

    Abcam Scientific Support

    回复于 Dec 21 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
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