Anti-LCLAT1抗体(ab122197)
Key features and details
- Rabbit polyclonal to LCLAT1
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LCLAT1抗体
参阅全部 LCLAT1 一抗 -
描述
兔多克隆抗体to LCLAT1 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 231-307 (LHPRTTGFTF VVDRLREGKN LDAVHDITVA YPHNIPQSEK HLLQGDFPRE IHFHVHRYPI DTLPTSKEDL QLWCHKR) of Human LCLAT1.
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阳性对照
- IHC-P: Human heart muscle, duodenum, testis, and fallopian tube tissues; ICC: Human cell line A-431.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122197于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages. -
组织特异性
Expressed at higher level in heart, kidney and pancreas than in brain, spleen, liver, lung, small intestine and placenta. -
通路
Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3. -
序列相似性
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. -
结构域
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 253558 Human
- Omim: 614241 Human
- SwissProt: Q6UWP7 Human
- Unigene: 468048 Human
- Unigene: 662770 Human
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别名
- 1-acylglycerol-3-phosphate O-acyltransferase 8 antibody
- 1-AGP acyltransferase 8 antibody
- 1-AGPAT 8 antibody
see all
图片
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Immunocytochemistry - Anti-LCLAT1 antibody (ab122197)
Immunocytochemistry/immunofluorescence analysis of human cell line A-431 shows labelling LCLAT1 with ab122197 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCLAT1 antibody (ab122197)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling LCLAT1 with ab122197 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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