AB51060

重组Anti-LCAT抗体[EPR1384Y]

Anti-LCAT antibody [EPR1384Y]

BOND RX™ Validated
RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal LCAT antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications.

查看别名

Phosphatidylcholine-sterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, LCAT

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (AB51060)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (AB51060)

ab51060 (unpurified) at 1/100 dilution staining LCAT in paraffin embedded human brain tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LCAT antibody [EPR1384Y] (AB51060)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling LCAT with purified ab51060 at 1/100 dilution (1.03 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Lab

Western blot - Anti-LCAT antibody [EPR1384Y] (AB51060)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Exposure time : Lane 1-3, 7-9 : 180 seconds; Lane 4-6 : 140 seconds

Low expression : HEK 293 (PMID : 7721870).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

ab109417 is more sensitive than ab51060 in WB testing.

Lanes 1 - 6:

Western blot - Anti-LCAT antibody [EPR1384Y] (ab51060) at 1/1000 dilution

Lanes 7 - 9:

Western blot - Anti-LCAT antibody [EPR1383(2)] (<a href='/products/primary-antibodies/lcat-antibody-epr13832-ab109417'>ab109417</a>) at 1/1000 dilution

Lanes 1, 4 and 7:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 2, 5 and 8:

COS-1 (Cercopithecus aethiops kidney epithelial) whole cell lysate at 20 µg

Lanes 3, 6 and 9:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 62 kDa

false

不同偶联物与剂型 (2)

Carrier free

Anti-LCAT antibody [EPR1384Y] - Low endotoxin, Azide free
Carrier free

Anti-LCAT antibody [EPR1384Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1384Y

亚型

IgG

不含载体蛋白

反应种属

Human, African green monkey

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "The immunostaining was performed on a Leica Biosystems BOND® RX instrument.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1/100.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LCAT also known as lecithin-cholesterol acyltransferase is an enzyme with a molecular mass of approximately 68 kDa. It belongs to the family of esterases and is mainly expressed in the liver. LCAT circulates in the bloodstream playing a significant role in lipid metabolism by catalyzing the formation of cholesteryl esters from free cholesterol and phosphatidylcholine (lecithin) on the surface of high-density lipoproteins (HDL).

Biological function summary

This enzyme functions centrally in the maturation of high-density lipoprotein particles. LCAT interacts with and acts upon HDL converting nascent HDL into mature HDL by catalyzing the esterification of cholesterol. This process results in the sequestration of cholesterol within the core of HDL particles essential for reverse cholesterol transport to the liver. Moreover LCAT does not operate as part of a larger complex but is an active enzyme on its own within various lipid transport processes.

Pathways

LCAT's action significantly influences the lipid metabolism and reverse cholesterol transport pathways. In the lipid metabolism pathway it works closely with apolipoproteins such as apoA-I which serves as an activator. ApoA-I stabilizes the enzymatic action of LCAT promoting cholesterol homeostasis whereas LCAT ensures the efficient transfer of cholesterol to the liver within the reverse cholesterol transport pathway contributing to the maintenance of lipid balance.

LCAT deficiency can lead to conditions like familial LCAT deficiency and Fish Eye disease. These disorders are marked by abnormal cholesterol and lipid accumulation due to impaired LCAT activity. Apolipoproteins particularly apoA-I also demonstrate a significant connection as mutations or lack of apoA-I compromise LCAT activation exacerbating hyperlipidemia and related lipid disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed : 10329423, PubMed : 19065001, PubMed : 26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16 : 0-18 : 2 or 18 : O-18 : 2 phosphatidylcholines (PubMed : 8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed : 10722751). Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed : 8016111). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (PubMed : 8016111). Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters (PubMed : 24620755).

See full target information LCAT

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of atherosclerosis and thrombosis 30:1070-1082 PubMed36384970

2022

Two Cases of Acquired High-Density Lipoprotein Deficiency with Immunoglobulin G4-Related Lecithin-Cholesterol Acyltransferase Autoantibody.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohiro Komatsu,Yuka Katsurada,Kazuya Miyashita,Satomi Abe,Takafumi Nishida,Yasuhiro Endo,Manami Teramoto,Kei Sasaki,Junko Arakawa,Makoto Sasaki,Natsuko Suzuki,Koji Kuwata,Toshihiko Imakiire,Takayuki Miyake,Masami Sakurada,Susumu Matsukuma,Tsutomu Hirano,Yoshinari Uehara,Katsunori Ikewaki

Heliyon 8:e11271 PubMed36387451

2022

First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Aso,Tokuo T Yamamoto,Masayuki Kuroda,Jun Wada,Yoshitaka Kubota,Ko Ishikawa,Yoshiro Maezawa,Naoya Teramoto,Ayako Tawada,Sakiyo Asada,Yasuyuki Aoyagi,Mika Kirinashizawa,Akinobu Onitake,Yuta Matsuura,Kunio Yasunaga,Shun-Ichi Konno,Katsuaki Nishino,Misato Yamamoto,Junko Miyoshi,Norihiko Kobayashi,Masami Tanio,Takayuki Ikeuchi,Hidetoshi Igari,Nobuyuki Mitsukawa,Hideki Hanaoka,Koutaro Yokote,Yasushi Saito

Yonsei medical journal 61:780-788 PubMed32882762

2020

MiR-542-5p Inhibits Hyperglycemia and Hyperlipoidemia by Targeting FOXO1 in the Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Tian,Hui Min Ying,Yuan Yuan Wang,Bo Ning Cheng,Juan Chen

Biomolecules 9: PubMed31779197

2019

LCAT, ApoD, and ApoA1 Expression and Review of Cholesterol Deposition in the Cornea.

Applications

Unspecified application

Species

Unspecified reactive species

Rhonda Flores,Xueting Jin,Janet Chang,Connie Zhang,David G Cogan,Ernst J Schaefer,Howard S Kruth

Journal of lipid research 57:1059-73 PubMed27112635

2016

ApoL1 levels in high density lipoprotein and cardiovascular event presentation in patients with familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Judit Cubedo,Teresa Padró,Rodrigo Alonso,Pedro Mata,Lina Badimon

Reproduction (Cambridge, England) 139:163-76 PubMed19776099

2009

The reverse cholesterol transport system as a potential mediator of luteolysis in the primate corpus luteum.

Applications

Unspecified application

Species

Unspecified reactive species

Randy L Bogan,Jon D Hennebold

View all publications

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重组Anti-LCAT抗体[EPR1384Y]