AB222776

重组Anti-LC3B抗体[RM293]

Anti-LC3B antibody [RM293]

Recombinant
Lab Essentials
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(6 Publications)

Rabbit Recombinant Monoclonal LC3B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3B.

查看别名

MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, Microtubule-associated proteins 1A/1B light chain 3B, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (AB222776)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (AB222776)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated (left panel) or treated with chloroquine (right panel) stained for LC3B (red) using ab222776 at 1/200 dilution in ICC/IF.

Actin filaments were labeled with fluorescein phalloidin (green). Nuclei were labeled with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (AB222776)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (AB222776)

Formalin-fixed, paraffin-embedded human brain tissue stained for LC3B with ab222776 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LC3B antibody [RM293] (AB222776)

All lanes:

Western blot - Anti-LC3B antibody [RM293] (ab222776) at 1/200 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with chloroquine, cell lysate

Predicted band size: 15 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

RM293

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

Synthetic Peptide within Human MAP1LC3B. The exact immunogen used to generate this antibody is proprietary information.

Q9GZQ8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/200", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

ab222776 was purified from an animal origin–free culture supernatant.

存储溶液

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20220913 PubMed40226364

2025

Stigmasterol alleviates endplate chondrocyte degeneration through inducing mitophagy by enhancing PINK1 mRNA acetylation via the ESR1/NAT10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Li,Xiaofeng Chen,Baoci Huang,Junjie He,Junxian Xie,Weijun Guo,Jinjun Liang,Jiajian Ruan,Jincheng Liu,Zhen Xiang,Lixin Zhu

Cancer biology & therapy 26:2469927 PubMed40018990

2025

The RNA-binding protein ELAVL1 promotes Beclin1-mediated cellular autophagy and thus endometrial cancer development by affecting LncRNA-neat stability.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlu Luo,Xueyan Zhong,Xinzhao Sun,Jiangtao Fan

Oral diseases 29:2837-2844 PubMed35770313

2022

Apocynin inhibits compressive force-induced apoptosis and autophagy of periodontal ligament stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Yuchun Shi,Yanbin Fu

Experimental and therapeutic medicine 22:1271 PubMed34594408

2021

TFEC contributes to cardiac hypertrophy by inhibiting AMPK/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhao,Zhenyu Wang,Yehong Chi,Chunmei Ni,Xudan Zheng

Molecular medicine reports 24: PubMed34109426

2021

Dexmedetomidine attenuates neuronal injury induced by cerebral ischemia‑reperfusion by regulating miR‑199a.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Zhu,Huatang Zhao,Wenshan Zhang,Xingang Ma,Ye Liu

Aging 12: PubMed33495420

2021

Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hongchang Li,Wen Xu,Zhihua Xia,Weiyan Liu,Gaofeng Pan,Junbin Ding,Jindong Li,Jianfa Wang,Xiaofeng Xie,Daowen Jiang

View all publications

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重组Anti-LC3B抗体[RM293]

Anti-LC3B antibody [RM293]

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (AB222776)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (AB222776)

Western blot - Anti-LC3B antibody [RM293] (AB222776)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原