Anti-LC3B 抗体 - N-terminal

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody - N-terminal (AB229327)

Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and treated HeLa cells labelling LC3B protein at autophagosome with ab229327 at a 1/500 dilution at RT for 15 min (Green). Phalloidin, a cytoskeleton marker was used and diluted at 1/200 dilution. The nuclear counter stain is DAPI.
Scale bar= 10 µm.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody - N-terminal (AB229327)

Immunofluorescence analysis of 4% paraformaldehyde fixed Mock and treated HepG2 cells labelling LC3B protein at vesicle with ab229327 at a 1/500 dilution at RT for 15 min (Green). Alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 dilution (Red). The nuclear counter stain is DAPI (Blue).

• Flow Cyt

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Flow Cytometry - Anti-LC3B antibody - N-terminal (AB229327)

Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling LC3B with ab229327 at 1/100 dilution (blue) compared with an unlabelled sample (brown).

Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody - N-terminal (AB229327)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, mock treated (left panel) or treated with 50 μM Chloroquine for 24 hr (right panel), stained for LC3B (green) using ab229327 at 1/2000 dilution in ICC/IF.

Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution.

Blue : Hoechst 33342 staining.

• IP

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Immunoprecipitation - Anti-LC3B antibody - N-terminal (AB229327)

LC3B was immunoprecipitated from U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract (1 mg for IP, 20% of IP loaded) with 5 μg ab229327. Western blot was performed from the immunoprecipitate using ab229327. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : U-87 MG whole cell extract.

Lane 2 : Control IgG IP in U-87 MG whole cell extract.

Lane 3 : ab229327 IP in U-87 MG whole cell extract.

All lanes:

Immunoprecipitation - Anti-LC3B antibody - N-terminal (ab229327)

Predicted band size: 15 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody - N-terminal (AB229327)

Paraffin-embedded rat cerebellum tissue stained for LC3B protein at cytoplasm with ab229327 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody - N-terminal (AB229327)

Paraffin-embedded rat brain tissue stained for LC3B protein at cytoplasm with ab229327 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody - N-terminal (AB229327)

Paraffin-embedded mouse cerebellum tissue stained for LC3B protein at cytoplasm with ab229327 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• WB

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Western blot - Anti-LC3B antibody - N-terminal (AB229327)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/2500 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

HeLa whole cell extract treated with 50μM Chloroquine for 24 hours at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

• WB

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Western blot - Anti-LC3B antibody - N-terminal (AB229327)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/1000 dilution

Lane 1:

NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell extract at 30 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 4:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-LC3B antibody - N-terminal (AB229327)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/1000 dilution

Lane 1:

Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 whole cell extract treated with 3 μM thapsigargin for 16 hours at 30 µg

Lane 3:

HepG2 whole cell extract treated with 3 μM thapsigargin for 24 hours at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

• WB

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Western blot - Anti-LC3B antibody - N-terminal (AB229327)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/500 dilution

Lane 1:

Untreated HUVEC whole cell extracts at 30 µg

Lane 2:

Treated HUVEC whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-LC3B antibody - N-terminal (AB229327)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/500 dilution

Lane 1:

Wild-type (WT) 293T cell extracts at 30 µg

Lane 2:

LC3B knockout (KO) 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-LC3B antibody - N-terminal (AB229327)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-LC3B antibody - N-terminal (AB229327)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/1000 dilution

All lanes:

Rat brain lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-LC3B antibody - N-terminal (AB229327)

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/1500 dilution

Lane 1:

Huh7 whole cell lysate (un-infected) at 20 µg

Lane 2:

Huh7 whole cell lysate (HCV-infected) at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

• WB

Supplier Data

Western blot - Anti-LC3B antibody - N-terminal (AB229327)

All lanes:

Western blot - Anti-LC3B antibody - N-terminal (ab229327) at 1/1500 dilution

Lane 1:

Huh7 whole cell lysate (untreated) at 20 µg

Lane 2:

Huh7 whole cell lysate (3 μM Thapsigargin treatment for 12 hr) at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false