AB243506

Anti-LC3B抗体[GT3612]

Name: LC3B抗体[GT3612] (ab243506)| Abcam中文官网
Brand: Abcam Limited
SKU: AB243506
Price: 4644 CNY
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Anti-LC3B antibody [GT3612]

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(25 Publications)

Mouse Monoclonal LC3B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human MAP1LC3B.

查看别名

MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, Microtubule-associated proteins 1A/1B light chain 3B, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [GT3612] (AB243506)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [GT3612] (AB243506)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, mock treated (left) and treated with 50 μM Chloroquine for 24 hr (right), stained for LC3B (green) using ab243506 at 1/200 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Scale bar = 10 μm.

Unknown

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts at 50 µg

Lane 2:

50μM Chloroquine (24 hr) treated HeLa whole cell extracts at 50 µg

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1:

Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts at 30 µg

Lane 2:

3 μM Thapsigargin (12 hr) treated HepG2 whole cell extracts at 30 µg

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

LC3A-transfected HEK-293T whole cell extracts at 30 µg

Lane 3:

LC3B-transfected HEK-293T whole cell extracts at 30 µg

Predicted band size: 15 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

GT3612

亚型

IgG2a

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human MAP1LC3B. The exact immunogen used to generate this antibody is proprietary information.

Q9GZQ8

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LC3B also known as microtubule-associated protein 1 light chain 3 beta plays an important role in autophagy a process that maintains cellular homeostasis. The LC3B protein with a molecular weight of approximately 14 kDa undergoes lipidation to form LC3-II which associates with autophagosomal membranes. This protein expresses ubiquitously in various tissues including liver muscle and brain. Researchers often detect LC3B using techniques like LC3B western blot and LC3B immunofluorescence due to its function as a marker indicating autophagy levels.

Biological function summary

LC3B contributes significantly to the formation and maturation of autophagosomes. LC3B part of the autophagy-related protein complex binds to autophagic membranes. During this process LC3-I converts to LC3-II a lipid-phosphatidylethanolamine conjugate essential for autophagosome membrane expansion and closure. This mechanism helps remove damaged organelles and misfolded proteins from cells therefore contributing to cellular quality control.

Pathways

LC3B integrates into the autophagy pathway which is critical for cellular adaptive responses to stress. The mammalian target of rapamycin (mTOR) pathway regulates autophagy where mTOR inhibition activates LC3B promoting autophagosome formation. Moreover LC3B operates alongside other proteins like Beclin-1 and ULK1 facilitating the initiation and progression of autophagy under nutrient starvation conditions. These interactions highlight LC3's role in cellular energy balance and survival mechanisms.

LC3B connects with conditions such as cancer and neurodegenerative diseases. Altered autophagy levels mediated by LC3B often associate with tumorigenesis where its dysregulation can affect cancer progression. Furthermore LC3B also links to neurodegenerative diseases like Alzheimer's where impaired autophagy disrupts neuronal function. LC3B interacts with proteins such as p62/SQSTM1 which affects protein aggregate clearance a critical factor in neurodegenerative pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 23209295, PubMed : 28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed : 22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed : 20418806, PubMed : 23209295, PubMed : 28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed : 24089205). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed : 31006537, PubMed : 31006538). Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy (PubMed : 30420355).

See full target information MAP1LC3B

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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed41062300

2025

Identification of KLHL35, WDR72, and WDR78 as Prognostic Biomarkers in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayou Ye,Tingting Zhang,Guangsheng Wang,Guofeng Bian,Aijun Chen,Xin Zhou

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran

Autophagy reports 4:2475527 PubMed40395986

2025

escapes LAPosome and modulates macrophage response in a xenophagy-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Reyne,Jeffrey Arrindell,Eloïne Bestion,Soraya Mezouar,Benoit Desnues

Journal of nanobiotechnology 23:287 PubMed40211295

2025

Adipose-derived small extracellular vesicle miR-146a-5p targets Fbx32 to regulate mitochondrial autophagy and delay aging in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Mengran Qin,Yan Wang,Zihan Wang,Benchao Dong,Peichuan Yang,Youyi Liu,Qianyun Xi,Jianxiong Ma

Journal of translational medicine 23:237 PubMed40016828

2025

FlaA N/C attenuates radiation-induced lung injury by promoting NAIP/NLRC4/ASC inflammasome autophagy and inhibiting pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shihua Deng,Yueyan Yang,Shuang He,Zixin Chen,Xun Xia,Ting Zhang,Qing Yin,Teng Liu,Dongming Wu,Kejian Pan,Ying Xu

Acta pharmaceutica Sinica. B 14:5435-5450 PubMed39807326

2025

Nanoengineered mitochondria enable ocular mitochondrial disease therapy the replacement of dysfunctional mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Nahui Liu,Lifan Hu,Jingsong Yang,Mengmeng Han,Tianjiao Zhou,Lei Xing,Hulin Jiang

Molecular neurodegeneration 19:91 PubMed39609868

2024

NOTCH2NLC GGC intermediate repeat with serine induces hypermyelination and early Parkinson's disease-like phenotypes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Tu,Xin Yi Yeo,Zhi-Wei Zhang,Wei Zhou,Jayne Yi Tan,Li Chi,Sook-Yoong Chia,Zhihong Li,Aik Yong Sim,Brijesh Kumar Singh,Dongrui Ma,Zhidong Zhou,Isabelle Bonne,Shuo-Chien Ling,Adeline S L Ng,Sangyong Jung,Eng-King Tan,Li Zeng

Cellular and molecular life sciences : CMLS 81:336 PubMed39120751

2024

Overexpression of long noncoding RNA DUXAP8 inhibits ER-phagy through activating AKT/mTOR signaling and contributes to preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hong Wei,Ling-Yun Liao,Yang-Xue Yin,Qin Xu,Shuang-Shuang Xie,Min Liu,Lin-Bo Gao,Hong-Qin Chen,Rong Zhou

International journal of molecular sciences 25: PubMed38892363

2024

Autophagy Promotes Enrichment of Raft Components within Extracellular Vesicles Secreted by Human 2FTGH Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Manganelli,Luciana Dini,Stefano Tacconi,Simone Dinarelli,Antonella Capozzi,Gloria Riitano,Serena Recalchi,Tuba Rana Caglar,Federica Fratini,Roberta Misasi,Maurizio Sorice,Tina Garofalo

Biomedicines 12: PubMed38397924

2024

The Ala1186Val Variant Linked to Cytoplasmic Body Myopathy and Cardiomyopathy Causes Protein Instability.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Onnée,Audrey Bénézit,Sultan Bastu,Aleksandra Nadaj-Pakleza,Béatrice Lannes,Flavie Ader,Corinne Thèze,Pascal Cintas,Claude Cances,Robert-Yves Carlier,Corinne Metay,Mireille Cossée,Edoardo Malfatti

View all publications

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Anti-LC3B抗体[GT3612]

Anti-LC3B antibody [GT3612]

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [GT3612] (AB243506)

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

Western blot - Anti-LC3B antibody [GT3612] (AB243506)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原