Anti-LC3B 抗体

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (AB51520)

ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
1) Control
2) Temozolomide

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (AB51520)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (AB51520)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LC3B antibody (AB51520)

Flow cytometry (Intracellular) analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (AB51520)

ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
1) Control
2) Temozolomide

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (AB51520)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (AB51520)

ab51520 at 1/2000 dilution staining treated U373-MG cells;
1) Control
2) C₂-ceramide.
The nuclei were stained with DAPI.

• WB

Lab

Western blot - Anti-LC3B antibody (AB51520)

False colour image of Western blot : Anti-LC3B antibody staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab63817 was shown to bind specifically to LC3B. A band was observed at 16/14 kDa (yellow arrows) in treated wild-type HepG2 cell lysates with no signal observed at this size in MAP1LC3B knockout cell line ab277828 (knockout cell lysate ab283796). To generate this image, wild-type and MAP1LC3B knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LC3B antibody (ab51520) at 1/3000 dilution

Lane 1:

Wild-type HepG2 untreated control cell lysate at 20 µg

Lane 2:

Wild-type HepG2 Treated Chloroquine (50 uM, 16 h) cell lysate at 20 µg

Lane 3:

MAP1LC3B knockout HepG2 untreated control cell lysate at 20 µg

Lanes 3 - 4:

Western blot - Human MAP1LC3B knockout Hep G2 cell line (<a href='/products/unavailable/human-map1lc3b-knockout-hep-g2-cell-line-ab277828'>ab277828</a>)

Lanes 3 - 4:

Western blot - Human MAP1LC3B knockout Hep G2 cell lysate (ab283796)

Lane 4:

MAP1LC3B knockout HepG2 Treated Chloroquine (50 uM, 16 h) cell lysate at 20 µg

Lane 5:

U-87 MG cell lysate at 20 µg

Lane 6:

PC-12 cell lysate at 20 µg

Predicted band size: 15 kDa

Observed band size: 14 kDa,16 kDa

false

• WB

Unknown

Western blot - Anti-LC3B antibody (AB51520)

All lanes:

Western blot - Anti-LC3B antibody (ab51520) at 1/3000 dilution

Lane 1:

Arsenic Trioxide at 0 µM treated U87-MG (human glioblastoma astrocytoma) lysates

Lane 2:

Arsenic Trioxide at 2 µM treated U87-MG (human glioblastoma astrocytoma) lysates

Lane 3:

Arsenic Trioxide at 4 µM treated U87-MG (human glioblastoma astrocytoma) lysates

Lane 4:

Arsenic Trioxide at 8 µM treated U87-MG (human glioblastoma astrocytoma) lysates

Predicted band size: 15 kDa

false

• WB

CiteAb

Western blot - Anti-LC3B antibody (AB51520)

Western Blotting using Anti-LC3B antibody, ab51520. Publication image from Nozawa, T. et al., 2020, Nat Commun, 32034138. Legend direct from paper.

TBC1D9 is involved in xenophagy during GAS infection.a, b Recruitment of the indicated proteins to invading GAS. a Representative confocal images and b quantification of GAS-positive cells. c, d Conversion of LC3-I to LC3-II. c Representative western blot of LC3 during starvation and GAS infection and d quantification of LC3-II/LC3-I ratio. e, f Localization of ULK1 in GAS-infected cells. e Representative confocal images of ULK1 recruitment to ubiquitin-coated GAS and f quantification of ULK1-positive GAS. g Intracellular bacterial CFU at 6 h p.i in WT and TBC1D9-KO HeLa cells. Scale bars, 10 µm. Data in (b) (n > 200 cells per condition), (d, f) (n > 50 bacteria per condition), and (g) represent the mean ± SEM of three independent experiments. P values calculated by two-tailed Student’s t test.

false