AB270325

重组Anti-LBP抗体[EPR22559-90] - BSA and Azide free (Detector)

Anti-LBP antibody [EPR22559-90] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal LBP antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

Lipopolysaccharide-binding protein, LBP, Lbp

1 Images

Sandwich ELISA - Anti-LBP antibody [EPR22559-90] - BSA and Azide free (Detector) (AB270325)

sELISA

Supplier Data

Sandwich ELISA - Anti-LBP antibody [EPR22559-90] - BSA and Azide free (Detector) (AB270325)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab269542).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22559-90

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab270325 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse LBP. The recommended pair for sandwich ELISA is:
Capture: ab270324, Mouse LBP Capture Antibody (unconjugated)
Detector: ab270325, Mouse LBP Detector Antibody (unconjugated)
The reference range value is 50 - 3200 ng/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.

Biological function summary

LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.

Pathways

LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.

Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed : 9144073). Acts as an affinity enhancer for CD14, facilitating its association with LPS (By similarity). Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed : 24380872).

See full target information Lbp

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重组Anti-LBP抗体[EPR22559-90] - BSA and Azide free (Detector)

Anti-LBP antibody [EPR22559-90] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-LBP antibody [EPR22559-90] - BSA and Azide free (Detector) (AB270325)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原