AB4476

Anti-LAT (phospho Y132)抗体

Name: LAT(磷酸化Y132)抗体 (ab4476)| Abcam中文官网
Brand: Abcam Limited
SKU: AB4476
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Anti-LAT (phospho Y132) antibody

(1 Review)

(17 Publications)

Rabbit Polyclonal LAT phospho Y132 antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human LAT phospho Y132.

查看别名

Linker for activation of T-cells family member 1, 36 kDa phosphotyrosine adapter protein, p36-38, pp36, LAT

1 Images

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Western blot - Anti-LAT (phospho Y132) antibody (AB4476)

Peptide Competition
Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.35 μg/ml phospho LAT (Tyr 132) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with : no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho LAT (Tyr 132) completely blocks the antibody signal, thereby demonstrating the specificity of the antibody, and stimulation-induced tyrosine phosphorylation of phospho LAT (Tyr 132).

All lanes:

Western blot - Anti-LAT (phospho Y132) antibody (ab4476)

Predicted band size: 28 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human LAT phospho Y132. The exact immunogen used to generate this antibody is proprietary information.

O43561

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated LAT. The final product is generated byaffinity chromatography using an LAT-derived peptide that is phosphorylated at tyrosine 132.

存储溶液

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.

Biological function summary

LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.

Pathways

LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.

LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development (PubMed : 23514740, PubMed : 25907557). Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

See full target information LAT phospho Y132

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37796108

2023

Allosteric inhibition of the T cell receptor by a designed membrane ligand.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Ye,Shumpei Morita,Justin J Chang,Patrick M Buckley,Kiera B Wilhelm,Daniel DiMaio,Jay T Groves,Francisco N Barrera

Theranostics 12:3316-3328 PubMed35547746

2022

NAD-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Woo Kim,Ga-Hee Ryoo,Hyun-Young Jang,So-Young Rah,Dong Hyun Lee,Do-Kyun Kim,Eun Ju Bae,Byung-Hyun Park

Frontiers in immunology 12:656366 PubMed34149695

2021

Identification and Characterization of the Amphioxus Lck and Its Associated Tyrosine Phosphorylation-Dependent Inhibitory LRR Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatao Zhou,Zhihui Xiao,Yanli Zhan,Xuemei Qu,Sisi Mou,Chong Deng,Tianxiang Zhang,Xin Lan,Shengfeng Huang,Yingqiu Li

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:8959-8974 PubMed32469452

2020

PTPN22 interacts with EB1 to regulate T-cell receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Zhang,Yang Yu,Bin Bai,Tao Wang,Jiahui Zhao,Na Zhang,Yanjiao Zhao,Xipeng Wang,Bing Wang

Frontiers in immunology 10:314 PubMed30873169

2019

T Cell Receptor (TCR)-Induced PLC-γ1 Sumoylation via PIASxβ and PIAS3 SUMO E3 Ligases Regulates the Microcluster Assembly and Physiological Function of PLC-γ1.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Long Wang,Jia-Qi Liang,Bei-Ni Gong,Ji-Ji Xie,Yu-Ting Yi,Xin Lan,Yingqiu Li

Frontiers in immunology 9:115 PubMed29456532

2018

A Stretch of Negatively Charged Amino Acids of Linker for Activation of T-Cell Adaptor Has a Dual Role in T-Cell Antigen Receptor Intracellular Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mikel M Arbulo-Echevarria,Isaac Narbona-Sánchez,Cecilia M Fernandez-Ponce,Inmaculada Vico-Barranco,Mª Dolores Rueda-Ygueravide,Michael L Dustin,Arkadiusz Miazek,Mª Carmen Duran-Ruiz,Francisco García-Cózar,Enrique Aguado

Journal of cell science 130:1217-1223 PubMed28209780

2017

Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Eugenio Bustos-Morán,Noelia Blas-Rus,Noa Beatriz Martin-Cófreces,Francisco Sánchez-Madrid

Nature communications 7:11389 PubMed27091106

2016

Aurora A drives early signalling and vesicle dynamics during T-cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Noelia Blas-Rus,Eugenio Bustos-Morán,Ignacio Pérez de Castro,Guillermo de Cárcer,Aldo Borroto,Emilio Camafeita,Inmaculada Jorge,Jesús Vázquez,Balbino Alarcón,Marcos Malumbres,Noa B Martín-Cófreces,Francisco Sánchez-Madrid

The Journal of experimental medicine 213:155-65 PubMed26783323

2016

A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Y Chan,Divya Punwani,Theresa A Kadlecek,Morton J Cowan,Jean L Olson,Erin F Mathes,Uma Sunderam,Shu Man Fu,Rajgopal Srinivasan,John Kuriyan,Steven E Brenner,Arthur Weiss,Jennifer M Puck

European journal of immunology 44:3741-6 PubMed25251667

2014

NF-κB is crucial in proximal T-cell signaling for calcium influx and NFAT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Crystina C Bronk,Sean Yoder,Emily L Hopewell,Shengyu Yang,Esteban Celis,Xue-Zhong Yu,Amer A Beg

View all publications

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Anti-LAT (phospho Y132)抗体

Anti-LAT (phospho Y132) antibody

Western blot - Anti-LAT (phospho Y132) antibody (AB4476)